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. 1982 Nov;38(2):449–454. doi: 10.1128/iai.38.2.449-454.1982

Monoclonal antibodies against Vibrio cholerae lipopolysaccharide.

B Gustafsson, A Rosén, T Holme
PMCID: PMC347760  PMID: 6183214

Abstract

A cell line producing monoclonal antibodies directed against the core region of Vibrio cholerae lipopolysaccharide has been established. These antibodies were inhibited by lipopolysaccharide preparations of both O-group 1 vibrios and some non-O-group 1 vibrios as detected in enzyme-linked immunosorbent assay-inhibition experiments. Coagglutination experiments with monoclonal and polyclonal antibodies adsorbed to protein A-carrying staphylococci were performed. All V. cholerae strains tested, regardless of serotype, were agglutinated when mixed with staphylococci coated with the monoclonal antibodies, whereas staphylococci coated with group-specific (O1) polyclonal antibodies only agglutinated with O-group 1 vibrios.

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Selected References

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