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. 1980 Oct;77(10):6047–6051. doi: 10.1073/pnas.77.10.6047

Regulation of Tn5 transposition in Salmonella typhimurium.

D Biek, J R Roth
PMCID: PMC350210  PMID: 6255475

Abstract

The drug-resistance element Tn5 transposes with high frequency immediately after entry into a cell. Establishment of Tn5 within a cell results in a decrease in this transposition frequency. This phenomenon resembles "zygotic induction" of repressible operons and prophages. Evidence is presented that Tn5 transposition is under negative control by a factor encoded within the element itself. Established Tn5 elements (that contain point mutations inactivating the resistance gene) are able to inhibit transposition of an incoming Tn5 element by a factor of 12- to 70-fold. Several deletion derivatives of Tn5 lack the ability to inhibit transposition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R. P., Roth J. R. Gene duplication in bacteria: alteration of gene dosage by sister-chromosome exchanges. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1083–1087. doi: 10.1101/sqb.1979.043.01.120. [DOI] [PubMed] [Google Scholar]
  2. Berg D. E., Davies J., Allet B., Rochaix J. D. Transposition of R factor genes to bacteriophage lambda. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3628–3632. doi: 10.1073/pnas.72.9.3628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berkowitz D., Hushon J. M., Whitfield H. J., Jr, Roth J., Ames B. N. Procedure for identifying nonsense mutations. J Bacteriol. 1968 Jul;96(1):215–220. doi: 10.1128/jb.96.1.215-220.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chou J., Casadaban M. J., Lemaux P. G., Cohen S. N. Identification and characterization of a self-regulated repressor of translocation of the Tn3 element. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4020–4024. doi: 10.1073/pnas.76.8.4020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gill R., Heffron F., Dougan G., Falkow S. Analysis of sequences transposed by complementation of two classes of transposition-deficient mutants of Tn3. J Bacteriol. 1978 Nov;136(2):742–756. doi: 10.1128/jb.136.2.742-756.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heffron F., McCarthy B. J., Ohtsubo H., Ohtsubo E. DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3. Cell. 1979 Dec;18(4):1153–1163. doi: 10.1016/0092-8674(79)90228-9. [DOI] [PubMed] [Google Scholar]
  7. Heffron F., So M., McCarthy B. J. In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6012–6016. doi: 10.1073/pnas.75.12.6012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hong J. S., Ames B. N. Localized mutagenesis of any specific small region of the bacterial chromosome. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3158–3162. doi: 10.1073/pnas.68.12.3158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hoppe I., Johnston H. M., Biek D., Roth J. R. A refined map of the hisG gene of Salmonella typhimurium. Genetics. 1979 May;92(1):17–26. doi: 10.1093/genetics/92.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. JACOB F., WOLLMAN E. Induction spontanée du développement du bactériophage lambda au cours de la recombinaison génétique, chez Escherichia coli K 12. C R Hebd Seances Acad Sci. 1954 Jul 19;239(3):317–319. [PubMed] [Google Scholar]
  11. Kleckner N., Ross D. G. Translocation and other recombination events involving the tetracycline-resistance element Tn10. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1233–1246. doi: 10.1101/sqb.1979.043.01.140. [DOI] [PubMed] [Google Scholar]
  12. Kleckner N. Translocatable elements in procaryotes. Cell. 1977 May;11(1):11–23. doi: 10.1016/0092-8674(77)90313-0. [DOI] [PubMed] [Google Scholar]
  13. Meyer R., Boch G., Shapiro J. Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance element. Mol Gen Genet. 1979 Mar 9;171(1):7–13. doi: 10.1007/BF00274009. [DOI] [PubMed] [Google Scholar]
  14. PARDEE A. B., JACOB F., MONOD J. Sur l'expression et le rôle des allèles inductible et constitutif dans la synthèse de la beta-galactosidase chez des zygotes d'Escherichia coli. C R Hebd Seances Acad Sci. 1958 May 28;246(21):3125–3128. [PubMed] [Google Scholar]
  15. Robinson M. K., Bennett P. M., Richmond M. H. Inhibition of TnA translocation by TnA. J Bacteriol. 1977 Jan;129(1):407–414. doi: 10.1128/jb.129.1.407-414.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rothstein S. J., Jorgensen R. A., Postle K., Reznikoff W. S. The inverted repeats of Tn5 are functionally different. Cell. 1980 Mar;19(3):795–805. doi: 10.1016/s0092-8674(80)80055-9. [DOI] [PubMed] [Google Scholar]
  17. Schmieger H. Phage P22-mutants with increased or decreased transduction abilities. Mol Gen Genet. 1972;119(1):75–88. doi: 10.1007/BF00270447. [DOI] [PubMed] [Google Scholar]
  18. Starlinger P. IS elements and transposons. Plasmid. 1980 May;3(3):241–259. doi: 10.1016/0147-619x(80)90039-6. [DOI] [PubMed] [Google Scholar]
  19. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]

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