Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1979 Jul;16(1):19–27. doi: 10.1128/aac.16.1.19

Isolation and Properties of Metronidazole-Resistant Mutants of Bacteroides fragilis

M L Britz 1,†, R G Wilkinson 1
PMCID: PMC352781  PMID: 475371

Abstract

Metronidazole-resistant mutants of Bacteroides fragilis, isolated after mutagenesis, had diminished ability to take up and metabolize the drug. All the metronidazole-resistant strains had depressed levels of pyruvate dehydrogenase compared with parent cultures. Their end products of glucose metabolism also differed from normal B. fragilis products and were consistent with deficiencies in pyruvate dehydrogenase activity.

Full text

PDF
27

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. An evaluation of metronidazole in the prophylaxis and treatment of anaerobic infections in surgical patients. J Antimicrob Chemother. 1975 Dec;1(4):393–341. doi: 10.1093/jac/1.4.393. [DOI] [PubMed] [Google Scholar]
  2. Britz M. L., Wilkinson R. G. Chloramphenicol acetyltransferase of Bacteroides fragilis. Antimicrob Agents Chemother. 1978 Jul;14(1):105–111. doi: 10.1128/aac.14.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Britz M. L., Wilkinson R. G. Purification and properties of beta-lactamase from Bacteroides fragilis. Antimicrob Agents Chemother. 1978 Mar;13(3):373–382. doi: 10.1128/aac.13.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cadogan-Cowper G., Wilkinson R. G. Anaerobic bacteriology. Isolation and identification of Bacteroides and Fusobacterium from human sources. Aust J Exp Biol Med Sci. 1974 Dec;52(6):897–908. [PubMed] [Google Scholar]
  5. Deutsch G., Foster J. L., McFadzean J. A., Parnell M. Human studies with "high dose" metronidazole: a non-toxic radiosensitizer of hypoxic cells. Br J Cancer. 1975 Jan;31(1):75–80. doi: 10.1038/bjc.1975.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldring J., McNaught W., Scott A., Gillespie G. Prophylactic oral antimicrobial agents in elective colonic surgery. A controlled trial. Lancet. 1975 Nov 22;2(7943):997–1000. doi: 10.1016/s0140-6736(75)90289-5. [DOI] [PubMed] [Google Scholar]
  7. Ings R. M., McFadzean J. A., Ormerod W. E. The mode of action of metronidazole in Trichomonas vaginalis and other micro-organisms. Biochem Pharmacol. 1974 May 1;23(9):1421–1429. doi: 10.1016/0006-2952(74)90362-1. [DOI] [PubMed] [Google Scholar]
  8. Lindmark D. G., Müller M. Hydrogenosome, a cytoplasmic organelle of the anaerobic flagellate Tritrichomonas foetus, and its role in pyruvate metabolism. J Biol Chem. 1973 Nov 25;248(22):7724–7728. [PubMed] [Google Scholar]
  9. Macdonald I. A., Williams C. N., Mahony D. E., Christie W. M. NAD- and NADP-dependent 7alpha-hydroxysteroid dehydrogenases from bacteroides fragilis. Biochim Biophys Acta. 1975 Mar 28;384(1):12–24. doi: 10.1016/0005-2744(75)90091-1. [DOI] [PubMed] [Google Scholar]
  10. O'Brien R. W., Morris J. G. Effect of metronidazole on hydrogen production by Clostridium acetobutylicum. Arch Mikrobiol. 1972;84(3):225–233. doi: 10.1007/BF00425200. [DOI] [PubMed] [Google Scholar]
  11. Populaire P., Decouvelaere B., Lebreton G., Pascal S. Dosage colorimétrique de l'(hydroxy-2 éthyl)-1 méthyl-2-nitro-5 imidazole (métronidazole) Ann Pharm Fr. 1968 Jul-Aug;26(7):549–556. [PubMed] [Google Scholar]
  12. Ralph E. D., Kirby W. M. Unique bactericidal action of metronidazole against Bacteroides fragilis and Clostridium perfringens. Antimicrob Agents Chemother. 1975 Oct;8(4):409–414. doi: 10.1128/aac.8.4.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tally F. P., Goldin B. R., Sullivan N., Johnston J., Gorbach S. L. Antimicrobial activity of metronidazole in anaerobic bacteria. Antimicrob Agents Chemother. 1978 Mar;13(3):460–465. doi: 10.1128/aac.13.3.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tally F. P., Sutter V. L., Finegold S. M. Treatment of anaerobic infections with metronidazole. Antimicrob Agents Chemother. 1975 May;7(5):672–675. doi: 10.1128/aac.7.5.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Urtasun R. C., Sturmwind J., Rabin H., Band P. R., Chapman J. D. Letter: "High-dose" metronidazole: a preliminary pharmacological study prior to its investigational use in clinical radiotherapy trials. Br J Radiol. 1974 May;47(557):297–299. doi: 10.1259/0007-1285-47-557-297-c. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES