Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1979 Jun;30(3):891–898. doi: 10.1128/jvi.30.3.891-898.1979

Multiple copies of Shope virus DNA are present in cells of benign and malignant non-virus-producing neoplasms.

J G Stevens, F O Wettstein
PMCID: PMC353400  PMID: 225548

Abstract

In an initial efforts to characterize the virological basis of neoplasia in the Shope papilloma-carcinoma system, the extent to which the viral genome is present in non-virus-producing benign and malignant tumors in domestic rabbits was established. Employing nick-translated radioactive viral DNA purified from productively infected papillomas on cotton tail rabbits as a probe, it was found that (i) papillomas, primary carcinomas, and metastatic carcinomas contain 10 to about 100 copies of the viral genome per diploid cell equivalent of DNA and (ii) viral DNA is present in detectable amounts in essentially all neoplastic cells. These results are consistent with the suggestion that continued presence of the viral genome is necessary for induction and maintenance of malignant as well as benign neoplasms.

Full text

PDF
892

Images in this article

894Image
on p.894
896Image
on p.896

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Botchan M., Topp W., Sambrook J. The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell. 1976 Oct;9(2):269–287. doi: 10.1016/0092-8674(76)90118-5. [DOI] [PubMed] [Google Scholar]
  2. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  3. Cook M. L., Bastone V. B., Stevens J. G. Evidence that neurons harbor latent herpes simplex virus. Infect Immun. 1974 May;9(5):946–951. doi: 10.1128/iai.9.5.946-951.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crawford L. V. Nucleic acids of tumor viruses. Adv Virus Res. 1969;14:89–152. doi: 10.1016/s0065-3527(08)60558-8. [DOI] [PubMed] [Google Scholar]
  5. Evans C. A., Rashad A. L. Virus content of Shope papillomas of cottontail rabbits. Cancer Res. 1967 Jun;27(6):1011–1015. [PubMed] [Google Scholar]
  6. Haase A. T., Stowring L., Narayan P., Griffin D., Price D. Slow persistent infection caused by visna virus: role of host restriction. Science. 1977 Jan 14;195(4274):175–177. doi: 10.1126/science.188133. [DOI] [PubMed] [Google Scholar]
  7. ITO Y. Relationship of components of papilloma virus to papilloma and carcinoma cells. Cold Spring Harb Symp Quant Biol. 1962;27:387–394. doi: 10.1101/sqb.1962.027.001.036. [DOI] [PubMed] [Google Scholar]
  8. Lancaster W. D., Olson C., Meinke W. Quantitation of bovine papilloma viral DNA in viral-induced tumors. J Virol. 1976 Mar;17(3):824–831. doi: 10.1128/jvi.17.3.824-831.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Murray K., Murray N. E. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. J Mol Biol. 1975 Nov 5;98(3):551–564. doi: 10.1016/s0022-2836(75)80086-6. [DOI] [PubMed] [Google Scholar]
  11. NOYES W. F., MELLORS R. C. Fluorescent antibody detection of the antigens of the Shope papilloma virus in papillomas of the wild and domestic rabbit. J Exp Med. 1957 Oct 1;106(4):555–562. doi: 10.1084/jem.106.4.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Orth G., Jeanteur P., Croissant O. Evidence for and localization of vegetative viral DNA replication by autoradiographic detection of RNA-DNA hybrids in sections of tumors induced by Shope papilloma virus. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1876–1880. doi: 10.1073/pnas.68.8.1876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ozanne B., Sharp P. A., Sambrook J. Transcription of simian virus 40. II. Hybridization of RNA extracted from different lines of transformed cells to the separated strands of simian virus 40 DNA. J Virol. 1973 Jul;12(1):90–98. doi: 10.1128/jvi.12.1.90-98.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. ROGERS S., KIDD J. G., ROUS P. Relationships of the Shope papilloma virus to the cancers it determines in domestic rabbits. Acta Unio Int Contra Cancrum. 1960;16:129–130. [PubMed] [Google Scholar]
  15. Radloff R., Bauer W., Vinograd J. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1514–1521. doi: 10.1073/pnas.57.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rashad A. L., Evans C. A. A difference in sites of DNA synthesis in virus-induced (Shope) and in chemically induced epidermal tumors of rabbit skin. Cancer Res. 1967 Sep;27(9):1639–1647. [PubMed] [Google Scholar]
  17. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  18. Sambrook J., Sharp P. A., Keller W. Transcription of Simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells. J Mol Biol. 1972 Sep 14;70(1):57–71. doi: 10.1016/0022-2836(72)90163-5. [DOI] [PubMed] [Google Scholar]
  19. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  20. Stevens J. G. Latent characteristics of selected herpesviruses. Adv Cancer Res. 1978;26:227–256. doi: 10.1016/s0065-230x(08)60089-5. [DOI] [PubMed] [Google Scholar]
  21. Wolf H. A procedure for simultaneous preparation of large amounts of DNA and RNA by the use of potassium iodide gradients. Anal Biochem. 1975 Oct;68(2):505–511. doi: 10.1016/0003-2697(75)90645-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES