Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1978 Jan;25(1):97–103. doi: 10.1128/jvi.25.1.97-103.1978

Adenovirus type 2 mRNA in transformed cells: map positions and difference in transport time.

M C Wilson, S G Sawicki, M Salditt-Georgieff, J E Darnell
PMCID: PMC353905  PMID: 621789

Abstract

Adenovirus type 2 rat transformed cells produced two polyadenylic acid-terminated mRNA's with approximate coordinates 1.5-4.4 and 4.4-11.0 on the physical map of the adenovirus type 2 genome. These mRNA's were also formed early during lytic infection in addition to one or more smaller mRNA's from the 4.4-11.0 region. In transformed cells, the 1.5-4.4 mRNA appeared in the cell cytoplasm without detectable lag, whereas the 4.4-11.0 mRNA required at least 20 to 30 min for the maximal rate of accumulation.

Full text

PDF
100

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachenheimer S., Darnell J. E. Hybridization of mRNA from adenovirus-transformed cells to segments of the adenovirus genome. J Virol. 1976 Jul;19(1):286–289. doi: 10.1128/jvi.19.1.286-289.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Büttner W., Veres-Molnár Z., Green M. Preparative isolation and mapping of adenovirus 2 early messenger RNA species. J Mol Biol. 1976 Oct 25;107(2):93–114. doi: 10.1016/s0022-2836(76)80020-4. [DOI] [PubMed] [Google Scholar]
  3. Chinnadurai G., Rho H. M., Horton R. B., Green M. mRNA from the transforming segment of the adenovirus 2 genome in productively infected and transformed cells. J Virol. 1976 Oct;20(1):255–263. doi: 10.1128/jvi.20.1.255-263.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chow L. T., Roberts J. M., Lewis J. B., Broker T. R. A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybrids. Cell. 1977 Aug;11(4):819–836. doi: 10.1016/0092-8674(77)90294-x. [DOI] [PubMed] [Google Scholar]
  5. Craig E. A. Analysis of early adenovirus 2 RNA using Eco R-R1 viral DNA fragments. J Virol. 1975 May;15(5):1202–1213. doi: 10.1128/jvi.15.5.1202-1213.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Craig E. A., McGrogan M., Mulder C., Raskas H. J. Identification of early adenovirus type 2 RNA species transcribed from the left-hand end of the genome. J Virol. 1975 Oct;16(4):905–912. doi: 10.1128/jvi.16.4.905-912.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Craig E. A., Raskas H. J. Two classes of cytoplasmic viral RNA synthesized early in productive infection with adenovirus 2. J Virol. 1974 Oct;14(4):751–757. doi: 10.1128/jvi.14.4.751-757.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Darnell J. E., Jr The origin of mRNA and the structure of the mammalian chromosome. Harvey Lect. 1973;(69):1–47. [PubMed] [Google Scholar]
  9. Evans R. M., Fraser N., Ziff E., Weber J., Wilson M., Darnell J. E. The initiation sites for RNA transcription in Ad2 DNA. Cell. 1977 Nov;12(3):733–739. doi: 10.1016/0092-8674(77)90273-2. [DOI] [PubMed] [Google Scholar]
  10. Flint J. The topography and transcription of the adenovirus genome. Cell. 1977 Feb;10(2):153–166. doi: 10.1016/0092-8674(77)90211-2. [DOI] [PubMed] [Google Scholar]
  11. Flint S. J., Gallimore P. H., Sharp P. A. Comparison of viral RNA sequences in adenovirus 2-transformed and lytically infected cells. J Mol Biol. 1975 Jul 25;96(1):47–68. doi: 10.1016/0022-2836(75)90181-3. [DOI] [PubMed] [Google Scholar]
  12. Gallimore P. H. Viral DNA in transformed cells. II. A study of the sequences of adenovirus 2 DNA IN NINE LINES OF TRANSFORMED RAT CELLS USING SPECIFIC FRAGMENTS OF THE VIRAL GENOME;. J Mol Biol. 1974 Oct 15;89(1):49–72. doi: 10.1016/0022-2836(74)90162-4. [DOI] [PubMed] [Google Scholar]
  13. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  14. Lewis J. B., Atkins J. F., Anderson C. W., Baum P. R., Gesteland R. F. Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1344–1348. doi: 10.1073/pnas.72.4.1344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lewis J. B., Atkins J. F., Baum P. R., Solem R., Gesteland R. F., Anderson C. W. Location and identification of the genes for adenovirus type 2 early polypeptides. Cell. 1976 Jan;7(1):141–151. doi: 10.1016/0092-8674(76)90264-6. [DOI] [PubMed] [Google Scholar]
  16. Lonberg-Holm K., Philipson L. Early events of virus-cell interaction in an adenovirus system. J Virol. 1969 Oct;4(4):323–338. doi: 10.1128/jvi.4.4.323-338.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nathans D., Smith H. O. Restriction endonucleases in the analysis and restructuring of dna molecules. Annu Rev Biochem. 1975;44:273–293. doi: 10.1146/annurev.bi.44.070175.001421. [DOI] [PubMed] [Google Scholar]
  18. Parsons J. T., Green M. Biochemical studies on adenovirus multiplication. 18. Resolution of early virus-specific RNA species in Ad 2 infected and transformed cells. Virology. 1971 Jul;45(1):154–162. doi: 10.1016/0042-6822(71)90122-x. [DOI] [PubMed] [Google Scholar]
  19. Philipson L., Pettersson U., Lindberg U., Tibbetts C., Vennström B., Persson T. RNA synthesis and processing in adenovirus-infected cells. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):447–456. doi: 10.1101/sqb.1974.039.01.057. [DOI] [PubMed] [Google Scholar]
  20. Sawicki S. G., Jelinek W., Darnell J. E. 3'-Terminal addition to HeLa cell nuclear and cytoplasmic poly (A). J Mol Biol. 1977 Jun 15;113(1):219–235. doi: 10.1016/0022-2836(77)90051-1. [DOI] [PubMed] [Google Scholar]
  21. Sharp P. A., Gallimore P. H., Flint S. J. Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):457–474. doi: 10.1101/sqb.1974.039.01.058. [DOI] [PubMed] [Google Scholar]
  22. Sharp P. A., Pettersson U., Sambrook J. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. J Mol Biol. 1974 Jul 15;86(4):709–726. doi: 10.1016/0022-2836(74)90348-9. [DOI] [PubMed] [Google Scholar]
  23. Shatkin A. J. Capping of eucaryotic mRNAs. Cell. 1976 Dec;9(4 Pt 2):645–653. doi: 10.1016/0092-8674(76)90128-8. [DOI] [PubMed] [Google Scholar]
  24. Tal J., Craig E. A., Zimmer S., Raskas H. J. Localization of adenovirus 2 messenger RNA's to segments of the viral genome defined by endonuclease R-R1. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4057–4061. doi: 10.1073/pnas.71.10.4057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wall R., Weber J., Gage Z., Darnell J. E. Production of viral mRNA in adenovirus- transformed cells by the post- transcriptional processing of heterogeneous nuclear RNA containing viral and cell sequences. J Virol. 1973 Jun;11(6):953–960. doi: 10.1128/jvi.11.6.953-960.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES