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. 1978 Jan;25(1):97–103. doi: 10.1128/jvi.25.1.97-103.1978

Adenovirus type 2 mRNA in transformed cells: map positions and difference in transport time.

M C Wilson, S G Sawicki, M Salditt-Georgieff, J E Darnell
PMCID: PMC353905  PMID: 621789

Abstract

Adenovirus type 2 rat transformed cells produced two polyadenylic acid-terminated mRNA's with approximate coordinates 1.5-4.4 and 4.4-11.0 on the physical map of the adenovirus type 2 genome. These mRNA's were also formed early during lytic infection in addition to one or more smaller mRNA's from the 4.4-11.0 region. In transformed cells, the 1.5-4.4 mRNA appeared in the cell cytoplasm without detectable lag, whereas the 4.4-11.0 mRNA required at least 20 to 30 min for the maximal rate of accumulation.

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Selected References

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  1. Bachenheimer S., Darnell J. E. Hybridization of mRNA from adenovirus-transformed cells to segments of the adenovirus genome. J Virol. 1976 Jul;19(1):286–289. doi: 10.1128/jvi.19.1.286-289.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Büttner W., Veres-Molnár Z., Green M. Preparative isolation and mapping of adenovirus 2 early messenger RNA species. J Mol Biol. 1976 Oct 25;107(2):93–114. doi: 10.1016/s0022-2836(76)80020-4. [DOI] [PubMed] [Google Scholar]
  3. Chinnadurai G., Rho H. M., Horton R. B., Green M. mRNA from the transforming segment of the adenovirus 2 genome in productively infected and transformed cells. J Virol. 1976 Oct;20(1):255–263. doi: 10.1128/jvi.20.1.255-263.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chow L. T., Roberts J. M., Lewis J. B., Broker T. R. A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybrids. Cell. 1977 Aug;11(4):819–836. doi: 10.1016/0092-8674(77)90294-x. [DOI] [PubMed] [Google Scholar]
  5. Craig E. A. Analysis of early adenovirus 2 RNA using Eco R-R1 viral DNA fragments. J Virol. 1975 May;15(5):1202–1213. doi: 10.1128/jvi.15.5.1202-1213.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Craig E. A., McGrogan M., Mulder C., Raskas H. J. Identification of early adenovirus type 2 RNA species transcribed from the left-hand end of the genome. J Virol. 1975 Oct;16(4):905–912. doi: 10.1128/jvi.16.4.905-912.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Craig E. A., Raskas H. J. Two classes of cytoplasmic viral RNA synthesized early in productive infection with adenovirus 2. J Virol. 1974 Oct;14(4):751–757. doi: 10.1128/jvi.14.4.751-757.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Darnell J. E., Jr The origin of mRNA and the structure of the mammalian chromosome. Harvey Lect. 1973;(69):1–47. [PubMed] [Google Scholar]
  9. Evans R. M., Fraser N., Ziff E., Weber J., Wilson M., Darnell J. E. The initiation sites for RNA transcription in Ad2 DNA. Cell. 1977 Nov;12(3):733–739. doi: 10.1016/0092-8674(77)90273-2. [DOI] [PubMed] [Google Scholar]
  10. Flint J. The topography and transcription of the adenovirus genome. Cell. 1977 Feb;10(2):153–166. doi: 10.1016/0092-8674(77)90211-2. [DOI] [PubMed] [Google Scholar]
  11. Flint S. J., Gallimore P. H., Sharp P. A. Comparison of viral RNA sequences in adenovirus 2-transformed and lytically infected cells. J Mol Biol. 1975 Jul 25;96(1):47–68. doi: 10.1016/0022-2836(75)90181-3. [DOI] [PubMed] [Google Scholar]
  12. Gallimore P. H. Viral DNA in transformed cells. II. A study of the sequences of adenovirus 2 DNA IN NINE LINES OF TRANSFORMED RAT CELLS USING SPECIFIC FRAGMENTS OF THE VIRAL GENOME;. J Mol Biol. 1974 Oct 15;89(1):49–72. doi: 10.1016/0022-2836(74)90162-4. [DOI] [PubMed] [Google Scholar]
  13. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  14. Lewis J. B., Atkins J. F., Anderson C. W., Baum P. R., Gesteland R. F. Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1344–1348. doi: 10.1073/pnas.72.4.1344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lewis J. B., Atkins J. F., Baum P. R., Solem R., Gesteland R. F., Anderson C. W. Location and identification of the genes for adenovirus type 2 early polypeptides. Cell. 1976 Jan;7(1):141–151. doi: 10.1016/0092-8674(76)90264-6. [DOI] [PubMed] [Google Scholar]
  16. Lonberg-Holm K., Philipson L. Early events of virus-cell interaction in an adenovirus system. J Virol. 1969 Oct;4(4):323–338. doi: 10.1128/jvi.4.4.323-338.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nathans D., Smith H. O. Restriction endonucleases in the analysis and restructuring of dna molecules. Annu Rev Biochem. 1975;44:273–293. doi: 10.1146/annurev.bi.44.070175.001421. [DOI] [PubMed] [Google Scholar]
  18. Parsons J. T., Green M. Biochemical studies on adenovirus multiplication. 18. Resolution of early virus-specific RNA species in Ad 2 infected and transformed cells. Virology. 1971 Jul;45(1):154–162. doi: 10.1016/0042-6822(71)90122-x. [DOI] [PubMed] [Google Scholar]
  19. Philipson L., Pettersson U., Lindberg U., Tibbetts C., Vennström B., Persson T. RNA synthesis and processing in adenovirus-infected cells. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):447–456. doi: 10.1101/sqb.1974.039.01.057. [DOI] [PubMed] [Google Scholar]
  20. Sawicki S. G., Jelinek W., Darnell J. E. 3'-Terminal addition to HeLa cell nuclear and cytoplasmic poly (A). J Mol Biol. 1977 Jun 15;113(1):219–235. doi: 10.1016/0022-2836(77)90051-1. [DOI] [PubMed] [Google Scholar]
  21. Sharp P. A., Gallimore P. H., Flint S. J. Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):457–474. doi: 10.1101/sqb.1974.039.01.058. [DOI] [PubMed] [Google Scholar]
  22. Sharp P. A., Pettersson U., Sambrook J. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. J Mol Biol. 1974 Jul 15;86(4):709–726. doi: 10.1016/0022-2836(74)90348-9. [DOI] [PubMed] [Google Scholar]
  23. Shatkin A. J. Capping of eucaryotic mRNAs. Cell. 1976 Dec;9(4 Pt 2):645–653. doi: 10.1016/0092-8674(76)90128-8. [DOI] [PubMed] [Google Scholar]
  24. Tal J., Craig E. A., Zimmer S., Raskas H. J. Localization of adenovirus 2 messenger RNA's to segments of the viral genome defined by endonuclease R-R1. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4057–4061. doi: 10.1073/pnas.71.10.4057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wall R., Weber J., Gage Z., Darnell J. E. Production of viral mRNA in adenovirus- transformed cells by the post- transcriptional processing of heterogeneous nuclear RNA containing viral and cell sequences. J Virol. 1973 Jun;11(6):953–960. doi: 10.1128/jvi.11.6.953-960.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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