Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1974 Feb;13(2):470–477. doi: 10.1128/jvi.13.2.470-477.1974

Purification of Single- and Double-Length M13 Virions by Polyacrylamide Gel Electrophoresis

Jacques Beaudoin 1,2,1, Timothy J Henry 1,2,2, David Pratt 1,2,3
PMCID: PMC355318  PMID: 4589859

Abstract

Complete separation of single- and double-length M13 virions from each other and from still-longer phage particles was achieved by electrophoresis of phage stocks through 2.5% polyacrylamide gels.

Full text

PDF
472

Images in this article

475Image
on p.475

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARBER W., MORSE M. L. HOST SPECIFICITY OF DNA PRODUCED BY ESCHERICHIA COLI. VI. EFFECTS ON BACTERIAL CONJUGATION. Genetics. 1965 Jan;51:137–148. doi: 10.1093/genetics/51.1.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRADLEY D. E. THE STRUCTURE OF SOME BACTERIOPHAGES ASSOCIATED WITH MALE STRAINS OF ESCHERICHIA COLI. J Gen Microbiol. 1964 Jun;35:471–482. doi: 10.1099/00221287-35-3-471. [DOI] [PubMed] [Google Scholar]
  3. Beaudoin J., Pratt D. Antiserum inactivation of electrophoretically purified M13 diploid virions: model for the F-specific filamentous bacteriophages. J Virol. 1974 Feb;13(2):466–469. doi: 10.1128/jvi.13.2.466-469.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Benzinger R. Restriction of infectious bacteriophage fd DNA's and an assay for in vitro host-controlled restriction and modification. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1294–1299. doi: 10.1073/pnas.59.4.1294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Caro L. G., Schnös M. The attachment of the male-specific bacteriophage F1 to sensitive strains of Escherichia coli. Proc Natl Acad Sci U S A. 1966 Jul;56(1):126–132. doi: 10.1073/pnas.56.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hjertén S., Jerstedt S., Tiselius A. Some aspects of the use of "continuous" and "discontinuous" buffer systems in polyacrylamide gel electrophoresis. Anal Biochem. 1965 May;11(2):219–223. doi: 10.1016/0003-2697(65)90008-4. [DOI] [PubMed] [Google Scholar]
  7. KELLENBERGER E., ARBER W. Electron microscopical studies of phage multiplication. I. A method for quantitative analysis of particle suspensions. Virology. 1957 Apr;3(2):245–255. doi: 10.1016/0042-6822(57)90091-0. [DOI] [PubMed] [Google Scholar]
  8. Lang D., Mitani M. Simplified quantitative electron microscopy of biopolymers. Biopolymers. 1970;9(3):373–379. doi: 10.1002/bip.1970.360090310. [DOI] [PubMed] [Google Scholar]
  9. Marvin D. A., Hohn B. Filamentous bacterial viruses. Bacteriol Rev. 1969 Jun;33(2):172–209. doi: 10.1128/br.33.2.172-209.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pattee P. A. Use of tetrazolium for improved resolution of bacteriophage plaques. J Bacteriol. 1966 Sep;92(3):787–788. doi: 10.1128/jb.92.3.787-788.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pratt D., Tzagoloff H., Beaudoin J. Conditional lethal mutants of the small filamentous coliphage M13. II. Two genes for coat proteins. Virology. 1969 Sep;39(1):42–53. doi: 10.1016/0042-6822(69)90346-8. [DOI] [PubMed] [Google Scholar]
  12. Pratt D., Tzagoloff H., Erdahl W. S. Conditional lethal mutants of the small filamentous coliphage M13. I. Isolation, complementation, cell killing, time of cistron action. Virology. 1966 Nov;30(3):397–410. doi: 10.1016/0042-6822(66)90118-8. [DOI] [PubMed] [Google Scholar]
  13. SALIVAR W. O., TZAGOLOFF H., PRATT D. SOME PHYSICAL-CHEMICAL AND BIOLOGICAL PROPERTIES OF THE ROD-SHAPED COLIPHAGE M13. Virology. 1964 Nov;24:359–371. doi: 10.1016/0042-6822(64)90173-4. [DOI] [PubMed] [Google Scholar]
  14. Salivar W. O., Henry T. J., Pratt D. Purification and properties of diploid particles of coliphage M13. Virology. 1967 May;32(1):41–51. doi: 10.1016/0042-6822(67)90250-4. [DOI] [PubMed] [Google Scholar]
  15. Simon E. H. The distribution and significance of multiploid virus particles. Prog Med Virol. 1972;14:36–67. [PubMed] [Google Scholar]
  16. Wiseman R. L., Dunker A. K., Marvin D. A. Filamentous bacterial viruses. 3. Physical and chemical characterization of the If1 virion. Virology. 1972 Apr;48(1):230–244. doi: 10.1016/0042-6822(72)90130-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES