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. 1972 Apr;9(4):611–620. doi: 10.1128/jvi.9.4.611-620.1972

Contact-Inhibited Revertant Cell Lines Isolated from Simian Virus 40-Transformed Cells III. Concanavalin A-Selected Revertant Cells

Lloyd A Culp a,1, Paul H Black a
PMCID: PMC356345  PMID: 4336561

Abstract

Contact-inhibited variants have been isolated by treatment of simian virus 40 (SV40)-transformed Balb/c 3T3 cells (SVT2) with the plant lectin concanavalin A. These con A revertant cells exhibit the following properties: (i) they resemble 3T3 cells morphologically and grow to saturation densities which are similar to that of 3T3 cells; (ii) they synthesize the SV40-specific T antigen and yield infectious virus after fusion with permissive monkey cells; (iii) they contain a high sialic acid content similar to that of 3T3 cells and not to that of SVT2 cells; sialic acid composition was found to be independent of serum concentration; (iv) they contain more chromosomes with the average number in the tetraploid range than the SVT2 cells from which they were derived; and (v) SVT2 and revertant cells, confluent or subconfluent, produce more collagen than Balb/3T3 cells. The relationship of surface membrane properties to contact inhibition of growth and the mechanisms for generating revertant cells are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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