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. 1972 Oct;10(4):648–652. doi: 10.1128/jvi.10.4.648-652.1972

Virus-Specific Ribonucleic Acid in the Nucleus and Cytoplasm of Rat Embryo Cells Transformed by Adenovirus Type 2

Koichiro Shimada a, Kei Fujinaga a,1, Seiji Hama a,2, Kenji Sekikawa a,1, Yohei Ito a
PMCID: PMC356515  PMID: 4673487

Abstract

Nuclei were isolated from rat embryo cells transformed by adenovirus type 2. Nuclear and cytoplasmic virus-specific ribonucleic acids (RNA) were characterized and quantitated by deoxyribonucleic acid (DNA)-RNA hybrid formation with adenovirus DNA. The results indicate that most, if not all, virus-specific RNA molecules are synthesized in the cell nucleus and subsequently transported into cytoplasm where they degrade with a half-life of 1 to 2 hr. No difference in base sequences between nuclear and cytoplasmic virus-specific RNA species can be detected by hybridization competition experiment with viral DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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