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. 1993 Mar;13(3):1903–1910. doi: 10.1128/mcb.13.3.1903

Epidermal growth factor regulates the exchange rate of guanine nucleotides on p21ras in fibroblasts.

L Buday 1, J Downward 1
PMCID: PMC359504  PMID: 8441421

Abstract

Treatment of intact Rat-1 fibroblasts with epidermal growth factor (EGF) leads to rapid activation of cellular ras-encoded proteins. By using the bacterial toxin streptolysin O to permeabilize these cells, it was shown that the low basal rate at which guanine nucleotides bind to, and dissociate from, ras-encoded protein in quiescent fibroblasts was greatly accelerated by EGF treatment. Nucleotide binding to other proteins was not affected. Stimulation of nucleotide exchange on ras-encoded protein required tyrosine kinase but not phospholipase activity. EGF had no effect on total GTPase-activating protein activity. Regulation of ras-encoded protein in Rat-1 fibroblasts is therefore mediated by stimulation, either directly or indirectly, of ras-encoded protein-specific guanine nucleotide exchange factors by the EGF receptor tyrosine kinase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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