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. 1991 Jan;11(1):175–181. doi: 10.1128/mcb.11.1.175

Mutational analysis of the functional domains of yeast K1 killer toxin.

H Zhu 1, H Bussey 1
PMCID: PMC359607  PMID: 1702512

Abstract

To determine the functional domains of K1 killer toxin, we analyzed the phenotypes of a set of mutations throughout regions encoding the alpha- and beta-toxin subunits that allow secretion of mutant toxins. A range of techniques have been used to examine the ability of mutant toxins to bind to beta-glucan cell wall receptor and to form lethal ion channels. Our results indicate that both the alpha and beta subunits are involved in beta-glucan receptor binding. Defects in ion channel formation and toxin immunity are confined to the hydrophobic alpha subunit of the toxin.

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Selected References

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