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. 1972 Dec;50(6):728–732. doi: 10.1104/pp.50.6.728

Metabolic Activities in Extracts of Mesophyll and Bundle Sheath Cells of Panicum miliaceum (L.) in Relation to the C4 Dicarboxylic Acid Pathway of Photosynthesis 1

Gerald E Edwards a, Maria Gutierrez a
PMCID: PMC366225  PMID: 16658252

Abstract

The activities of certain enzymes related to the carbon assimilation pathway in whole leaves, mesophyll cell extracts, and bundle sheath extracts of the C4 plant Panicum miliaceum have been measured and compared on a chlorophyll basis. Enzymes of the C4 dicarboxylic acid pathway—phosphoenolpyruvate carboxylase and NADP-malic dehydrogenase—were localized in mesophyll cells. Carbonic anhydrase was also localized in mesophyll cell extracts. Ribose 5-phosphate isomerase, ribulose 5-phosphate kinase, and ribulose diphosphate carboxylase—enzymes of the reductive pentose phosphate pathway—were predominantly localized in bundle sheath extracts. High activities of aspartate and alanine transaminases and glyceraldehyde-3-P dehydrogenase were found about equally distributed between the photosynthetic cell types. P. miliaceum had low malic enzyme activity in both mesophyll and bundle sheath extracts.

Isolated bundle sheath cells were capable of converting aspartate to oxalacetate at rates approaching the aspartate transaminase activity of bundle sheath extracts. The bundle sheath cells had a light induced CO2 fixation of 23 μmoles of CO2/mg chl·hr in the absence of exogenous substrates.

The photorespiratory enzymes, hydroxypyruvate reductase and glycolic oxidase, were about 3 fold higher in bundle sheath extracts than in mesophyll extracts when compared on a chlorophyll basis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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