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. 1973 Mar;51(3):468–473. doi: 10.1104/pp.51.3.468

Changes in Phospholipid Composition of a Winter Wheat Cultivar during Germination at 2 C and 24 C 1,2

Ian A de la Roche a, C J Andrews a, M Kates b
PMCID: PMC366289  PMID: 16658353

Abstract

Evaluation of various solvent systems for lipid extraction of wheat Triticum aestivum L. cv. Rideau seeds showed that boiling 2-propanol followed by the Bligh-Dyer procedure was the most efficient method, with respect to lipid yield and ability to inactivate lipolytic enzymes. Ten phospholipids were identified in dry seeds; the major components being phosphatidylcholine, lysophosphatidylcholine, N-acyl lysophosphatidyl-ethanolamine, N-acylphosphatidylethanolamine, and phosphatidylethanolamine. After growth for 1 week (2 C) or 31 hours (24 C), the proportions of phosphatidylethanolamine + lysophosphatidic acid and phosphatidic acid increased, lysophosphatidylcholine decreased, and the remaining phospholipids showed little change. At 5 weeks (2 C) or 72 hours (24 C), the seedlings showed 5-fold increases in the proportion of phosphatidic acid largely at the expense of phosphatidylcholine, small decreases in N-acyl lysophosphatidylethanolamine and N-acylphosphatidylethanolamine, and significant increases in lysophosphatidylcholine. The changes in phosphatidic acid and phosphatidylcholine are interpreted as being partially due to increasing phospholipase D activity during germination. In general, the phospholipid composition was similar in morphologically equivalent seedlings grown at 2 C or 24 C. The increased membrane content in seedlings grown at 2 C does not reflect any preferential synthesis of individual phospholipids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bomstein R. A. A new class of phosphatides isolated from soft wheat flour. Biochem Biophys Res Commun. 1965 Oct 8;21(1):49–54. doi: 10.1016/0006-291x(65)90424-9. [DOI] [PubMed] [Google Scholar]
  2. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  3. Dawson R. M., Clarke N., Quarles R. H. N-acylphosphatidylethanolamine, a phospholipid that is rapidly metabolized during the arly germnation of pea seeds. Biochem J. 1969 Sep;114(2):265–267. doi: 10.1042/bj1140265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dawson R. M. The formation of phosphatidylglycerol and other phospholipids by the transferase activity of phospholipase D. Biochem J. 1967 Jan;102(1):205–210. doi: 10.1042/bj1020205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Graveland A. Combination of thin layer chromatography and gas chromatography in the analysis on a microgram scale of lipids from wheat flour and wheat flour doughs. J Am Oil Chem Soc. 1968 Dec;45(12):834–840. doi: 10.1007/BF02540164. [DOI] [PubMed] [Google Scholar]
  6. Harris P., James A. T. The effect of low temperatures on fatty acid biosynthesis in plants. Biochem J. 1969 Apr;112(3):325–330. doi: 10.1042/bj1120325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Long C., Odavić R., Sargent E. J. The chemical nature of the products obtained by the action of cabbage-leaf phospholipase D on lysolecithin: the structure of lysolecithin. Biochem J. 1967 Jan;102(1):221–229. doi: 10.1042/bj1020221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Marshall M. O., Kates M. Biosynthesis of phosphatidylglycerol by cell-free preparations from spinach leaves. Biochim Biophys Acta. 1972 Apr 18;260(4):558–570. doi: 10.1016/0005-2760(72)90005-7. [DOI] [PubMed] [Google Scholar]
  9. Neskovic N. M., Kostic D. M. Quantitative analysis of rat liver phospholipids by a two-step thin-layer chromatographic procedure. J Chromatogr. 1968 Jun 4;35(2):297–300. doi: 10.1016/s0021-9673(01)82389-x. [DOI] [PubMed] [Google Scholar]
  10. Quarles R. H., Clarke N., Dawson R. M. Isolation of n-acyl phosphatidylethanolamine from pea seeds. Biochem Biophys Res Commun. 1968 Dec 30;33(6):964–968. doi: 10.1016/0006-291x(68)90407-5. [DOI] [PubMed] [Google Scholar]
  11. Quarles R. H., Dawson R. M. The distribution of phospholipase D in developing and mature plants. Biochem J. 1969 May;112(5):787–794. doi: 10.1042/bj1120787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. RICHARDSON T., TAPPEL A. L. Swelling of fish mitochondria. J Cell Biol. 1962 Apr;13:43–53. doi: 10.1083/jcb.13.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Redshaw E. S., Zalik S. Changes in lipids of cereal seedlings during vernalization. Can J Biochem. 1968 Sep;46(9):1093–1097. doi: 10.1139/o68-163. [DOI] [PubMed] [Google Scholar]
  14. Yang S. F., Freer S., Benson A. A. Transphosphatidylation by phospholipase D. J Biol Chem. 1967 Feb 10;242(3):477–484. [PubMed] [Google Scholar]

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