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. 1973 Apr;51(4):609–614. doi: 10.1104/pp.51.4.609

Regulation of β-Glucan Synthetase Activity by Auxin in Pea Stem Tissue

II. Metabolic Requirements 1

Peter M Ray a
PMCID: PMC366316  PMID: 16658380

Abstract

The 2- to 4-fold rise in particle-bound β-glucan synthetase (uridine diphosphate-glucose: β-1, 4-glucan glucosyltransferase) activity that can be induced by indoleacetic acid in pea stem tissue is not prevented by concentrations of actinomycin D or cycloheximide that inhibit growth and macromolecule synthesis. The rise is concluded to be a hormonally induced activation of previously existing, reversibly deactivated enzyme. The activation is not a direct allosteric effect of indoleacetic acid or sugars. It is blocked by inhibitors of energy metabolism, by 2-deoxyglucose, and by high osmolarity, but not by Ca2+ at concentrations that inhibit auxin-induced elongation and prevent promotion of sugar uptake by indoleacetic acid, and not by α, α′-dipyridyl at concentrations that inhibit formation of hydroxyproline. Regulation of the system could be due either to an ATP-dependent activating reaction affecting this enzyme, or to changes in levels of a primer or a lipid cofactor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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