Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1973 Oct;52(4):373–379. doi: 10.1104/pp.52.4.373

Activation and Inhibition of Ribulose 1,5-Diphosphate Carboxylase by 6-Phosphogluconate 1

Douglas K Chu a, J A Bassham a
PMCID: PMC366505  PMID: 16658565

Abstract

Ribulose 1,5-diphosphate carboxylase, when activated by preincubation with 1 mm bicarbonate and 10 mm MgCl2 in the absence of ribulose 1,5-diphosphate, remains activated for 20 minutes or longer after reaction is initiated by addition of ribulose diphosphate. If as little as 50 μm 6-phosphogluconate is added during this preincubation period, 5 minutes before the start of the reaction, a further 188% activation is observed. However, addition of 6-phosphogluconate at the same time or later than addition of ribulose diphosphate, or at any time with 50 mm bicarbonate, gives inhibition of the enzyme activity. Possible relevance of these effects in vivo regulatory effects is discussed.

Full text

PDF
379

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bassham J. A., Krause G. H. Free energy changes and metabolic regulation in steady-state photosynthetic carbon reduction. Biochim Biophys Acta. 1969 Oct 21;189(2):207–221. doi: 10.1016/0005-2728(69)90048-6. [DOI] [PubMed] [Google Scholar]
  2. Bassham J. A., Sharp P., Morris I. The effect of Mg2+ concentration on the pH optimum and Michaelis constants of the spinach chloroplast ribulosediphosphate carboxylase (carboxydismutase). Biochim Biophys Acta. 1968 May 28;153(4):898–900. doi: 10.1016/0005-2728(68)90019-4. [DOI] [PubMed] [Google Scholar]
  3. Buchanan B. B., Schürmann P. A regulatory mechanism for CO 2 assimilation in plant photosynthesis: activation of ribulose-1,5-diphosphate carboxylase by fructose 6-phosphate and deactivation by fructose 1,6-diphosphate. FEBS Lett. 1972 Jun 15;23(2):157–159. doi: 10.1016/0014-5793(72)80329-6. [DOI] [PubMed] [Google Scholar]
  4. Chu D. K., Bassham J. A. Inhibition of ribulose 1,5-diphosphate carboxylase by 6-phosphogluconate. Plant Physiol. 1972 Aug;50(2):224–227. doi: 10.1104/pp.50.2.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cooper T. G., Filmer D. The active species of "CO2" utilized by ribulose diphosphate carboxylase. J Biol Chem. 1969 Feb 10;244(3):1081–1083. [PubMed] [Google Scholar]
  6. Frieden C. Kinetic aspects of regulation of metabolic processes. The hysteretic enzyme concept. J Biol Chem. 1970 Nov 10;245(21):5788–5799. [PubMed] [Google Scholar]
  7. Jensen R. G., Bassham J. A. Photosynthesis by isolated chloroplasts. 3. Light activation of the carboxylation reaction. Biochim Biophys Acta. 1968 Jan 15;153(1):227–234. doi: 10.1016/0005-2728(68)90164-3. [DOI] [PubMed] [Google Scholar]
  8. Krause G. H., Bassham J. A. Induction of respiratory metabolism in illuminated Chlorella pyrenoidosa and isolated spinach chloroplasts by the addition of vitamin K5. Biochim Biophys Acta. 1969 Apr 8;172(3):553–565. doi: 10.1016/0005-2728(69)90151-0. [DOI] [PubMed] [Google Scholar]
  9. Kuehn G. D., McFadden B. A. Ribulose 1,5-diphosphate carboxylase from Hydrogenomonas eutropha and Hydrogenomonas facilis. I. Purification, metallic ion requirements, inhibition, and kinetic constants. Biochemistry. 1969 Jun;8(6):2394–2402. doi: 10.1021/bi00834a021. [DOI] [PubMed] [Google Scholar]
  10. Lin D. C., Novel P. S. Control of photosynthesis by Mg 2+ . Arch Biochem Biophys. 1971 Aug;145(2):622–632. doi: 10.1016/s0003-9861(71)80022-x. [DOI] [PubMed] [Google Scholar]
  11. Nobel P. S. Light-induced changes in the ionic content of chloroplasts in Pisum sativum. Biochim Biophys Acta. 1969 Jan 14;172(1):134–143. doi: 10.1016/0005-2728(69)90098-x. [DOI] [PubMed] [Google Scholar]
  12. PON N. G., RABIN B. R., CALVIN M. MECHANISM OF THE CARBOXYDISMUTASE REACTION. I. THE EFFECT OF PRELIMINARY INCUBATION OF SUBSTRATES, METAL ION AND ENZYME ON ACTIVITY. Biochem Z. 1963;338:7–19. [PubMed] [Google Scholar]
  13. Paulsen J. M., Lane M. D. Spinach ribulose diphosphate carboxylase. I. Purification and properties of the enzyme. Biochemistry. 1966 Jul;5(7):2350–2357. doi: 10.1021/bi00871a025. [DOI] [PubMed] [Google Scholar]
  14. Pedersen T. A., Kirk M., Bassham J. A. Inhibition of photophosphorylation and photosynthetic carbon cycle reactions by fatty acids and esters. Biochim Biophys Acta. 1966 Feb 7;112(2):189–203. doi: 10.1016/0926-6585(66)90320-7. [DOI] [PubMed] [Google Scholar]
  15. Rutner A. C. Estimation of the molecular weight of ribulose diphosphate carboxylase sub-units. Biochem Biophys Res Commun. 1970 Jun 5;39(5):923–929. doi: 10.1016/0006-291x(70)90412-2. [DOI] [PubMed] [Google Scholar]
  16. Rutner A. C., Lane M. D. Nonidentical subunits of ribulose diphosphate carboxylase. Biochem Biophys Res Commun. 1967 Aug 23;28(4):531–537. doi: 10.1016/0006-291x(67)90346-4. [DOI] [PubMed] [Google Scholar]
  17. Sugiyama T., Akazawa T. Subunit structure of spinach leaf ribulose 1,5-diphosphate carboxylase. Biochemistry. 1970 Nov 10;9(23):4499–4504. doi: 10.1021/bi00825a006. [DOI] [PubMed] [Google Scholar]
  18. Tabita F. R., McFadden B. A. Regulation of ribulose-1,5-diphosphate carboxylase by 6-phospho-D-gluconate. Biochem Biophys Res Commun. 1972 Sep 5;48(5):1153–1159. doi: 10.1016/0006-291x(72)90831-5. [DOI] [PubMed] [Google Scholar]
  19. WEISSBACH A., HORECKER B. L., HURWITZ J. The enzymatic formation of phosphoglyceric acid from ribulose diphosphate and carbon dioxide. J Biol Chem. 1956 Feb;218(2):795–810. [PubMed] [Google Scholar]
  20. Wildner G. F., Criddle R. S. Ribulose diphosphate carboxylase. I. A factor involved in light activation of the enzyme. Biochem Biophys Res Commun. 1969 Dec 4;37(6):952–960. doi: 10.1016/0006-291x(69)90223-x. [DOI] [PubMed] [Google Scholar]
  21. Wishnick M., Lane M. D., Scrutton M. C. The interaction of metal ions with ribulose 1,5-diphosphate carboxylase from spinach. J Biol Chem. 1970 Oct 10;245(19):4939–4947. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES