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. 1985 Mar;5(3):569–575. doi: 10.1128/mcb.5.3.569

Abundant nuclear ribonucleoprotein form of CAD RNA.

R Sperling, J Sperling, A D Levine, P Spann, G R Stark, R D Kornberg
PMCID: PMC366750  PMID: 3990684

Abstract

Transcripts of the CAD gene in Syrian hamster cells are as abundant in the nucleus as in the cytoplasm. This was shown by in situ hybridization of whole cells and by solution and blot hybridization of subcellular fractions. Similar results were obtained both for wild-type cells and for a mutant containing amplified CAD genes in which the level of CAD RNA is 150-fold greater. CAD nuclear RNA is indistinguishable from mature mRNA by gel electrophoresis and blot hybridization. Discrete higher-molecular-weight precursors are undetectable, although the persistence of a short length of intervening sequence in the otherwise fully processed RNA is not excluded. CAD RNA is released from nuclei by sonication in physiological conditions in a ribonucleoprotein form that sediments as a broad peak at about 200S in a sucrose gradient. CAD sequences extracted from nuclei by treatment with EDTA and RNase are found in the 30S particles previously described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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