Skip to main content
PMC home page
Clinical and Diagnostic Laboratory Immunology logoLink to Clinical and Diagnostic Laboratory Immunology
. 1994 Sep;1(5):487–492. doi: 10.1128/cdli.1.5.487-492.1994

Analysis of human antiviral cytotoxic T-lymphocyte responses for vaccine trials using cryopreserved mononuclear leukocytes: demonstration of feasibility with influenza virus-specific responses.

N el-Daher 1, J E Nichols 1, N J Roberts Jr 1
PMCID: PMC368318  PMID: 8556490

Abstract

The feasibility of measuring virus-specific human cytotoxic T-lymphocyte (CTL) activity by using cryopreserved mononuclear leukocytes to support clinical vaccine trials was addressed. Autologous fresh and cryopreserved cells from the same sample of peripheral blood were used as sources of CTL precursors and were tested for influenza virus-specific activity. The data indicated that virus-specific CTL activity could be measured by using cryopreserved cells; this could also be done in assays that are designed to characterize the responsible effector cell population.

Full text

PDF
489

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biddison W. E., Shaw S. Differences in HLA antigen recognition by human influenza virus-immune cytotoxic T cells. J Immunol. 1979 May;122(5):1705–1709. [PubMed] [Google Scholar]
  2. Biddison W. E., Ward F. E., Shearer G. M., Shaw S. The self determinants recognized by human virus-immune T cells can be distinguished from the serologically defined HLA antigens. J Immunol. 1980 Feb;124(2):548–552. [PubMed] [Google Scholar]
  3. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  4. Collins D. S., Findlay K., Harding C. V. Processing of exogenous liposome-encapsulated antigens in vivo generates class I MHC-restricted T cell responses. J Immunol. 1992 Jun 1;148(11):3336–3341. [PubMed] [Google Scholar]
  5. Ennis F. A., Rook A. H., Qi Y. H., Schild G. C., Riley D., Pratt R., Potter C. W. HLA restricted virus-specific cytotoxic T-lymphocyte responses to live and inactivated influenza vaccines. Lancet. 1981 Oct 24;2(8252):887–891. doi: 10.1016/s0140-6736(81)91389-1. [DOI] [PubMed] [Google Scholar]
  6. Hviid L., Albeck G., Hansen B., Theander T. G., Talbot A. A new portable device for automatic controlled-gradient cryopreservation of blood mononuclear cells. J Immunol Methods. 1993 Jan 4;157(1-2):135–142. doi: 10.1016/0022-1759(93)90079-m. [DOI] [PubMed] [Google Scholar]
  7. Keefer M. C., Bonnez W., Roberts N. J., Jr, Dolin R., Reichman R. C. Human immunodeficiency virus (HIV-1) gp160-specific lymphocyte proliferative responses of mononuclear leukocytes from HIV-1 recombinant gp160 vaccine recipients. J Infect Dis. 1991 Mar;163(3):448–453. doi: 10.1093/infdis/163.3.448. [DOI] [PubMed] [Google Scholar]
  8. Mbawuike I. N., Wyde P. R. Induction of CD8+ cytotoxic T cells by immunization with killed influenza virus and effect of cholera toxin B subunit. Vaccine. 1993 Sep;11(12):1205–1213. doi: 10.1016/0264-410x(93)90044-x. [DOI] [PubMed] [Google Scholar]
  9. McMichael A. J., Askonas B. A. Influenza virus-specific cytotoxic T cells in man; induction and properties of the cytotoxic cell. Eur J Immunol. 1978 Oct;8(10):705–711. doi: 10.1002/eji.1830081007. [DOI] [PubMed] [Google Scholar]
  10. McMichael A. HLA restriction of human cytotoxic T lymphocytes specific for influenza virus. Poor recognition of virus associated with HLA A2. J Exp Med. 1978 Dec 1;148(6):1458–1467. doi: 10.1084/jem.148.6.1458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Oldham R. K., Dean J. H., Cannon G. B., Ortaldo J. R., Dunston G., Applebaum F., McCoy J. L., Djeu J., Herberman R. B. Cryopreservation of human lymphocyte function as measured by in vitro assays. Int J Cancer. 1976 Aug 15;18(2):145–155. doi: 10.1002/ijc.2910180203. [DOI] [PubMed] [Google Scholar]
  12. Pantaleo G., De Maria A., Koenig S., Butini L., Moss B., Baseler M., Lane H. C., Fauci A. S. CD8+ T lymphocytes of patients with AIDS maintain normal broad cytolytic function despite the loss of human immunodeficiency virus-specific cytotoxicity. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4818–4822. doi: 10.1073/pnas.87.12.4818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reichman R. C., Strussenberg J., Green J. A. Herpes simplex virus-induced immunespecific interferon production by cryopreserved human peripheral blood mononuclear leukocytes. J Immunol Methods. 1986 Nov 6;93(2):241–246. doi: 10.1016/0022-1759(86)90196-1. [DOI] [PubMed] [Google Scholar]
  14. Roberts N. J., Jr, Douglas R. G., Jr, Simons R. M., Diamond M. E. Virus-induced interferon production by human macrophages. J Immunol. 1979 Jul;123(1):365–369. [PubMed] [Google Scholar]
  15. Roberts N. J., Jr, Steigbigel R. T. Effect of in vitro virus infection on response of human monocytes and lymphocytes to mitogen stimulation. J Immunol. 1978 Sep;121(3):1052–1058. [PubMed] [Google Scholar]
  16. Roberts N. J., Jr Variability of results of lymphocyte transformation assays in normal human volunteers. Responses of mononuclear leukocytes to mitogen stimulation. Am J Clin Pathol. 1980 Feb;73(2):160–164. doi: 10.1093/ajcp/73.2.160. [DOI] [PubMed] [Google Scholar]
  17. Rotteveel F. T., Braakman E., Robbe B., Lucas C. J. Recognition of influenza virus-infected B-cell lines by human influenza virus-specific CTL. Cell Immunol. 1988 Feb;111(2):473–481. doi: 10.1016/0008-8749(88)90110-4. [DOI] [PubMed] [Google Scholar]
  18. Shaw S., Shearer G. M., Biddison W. E. Human cytotoxic T-cell responses to type A and type B influenza viruses can be restricted by different HLA antigens. Implications for HLA polymorphism and genetic regulation. J Exp Med. 1980 Jan 1;151(1):235–245. doi: 10.1084/jem.151.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Strong D. M., Woody J. N., Factor M. A., Ahmed A., Sell K. W. Immunological responsiveness of frozen-thawed human lymphocytes. Clin Exp Immunol. 1975 Sep;21(3):442–455. [PMC free article] [PubMed] [Google Scholar]
  20. Townsend A. R., McMichael A. J. Specificity of cytotoxic T lymphocytes stimulated with influenza virus. Studies in mice and humans. Prog Allergy. 1985;36:10–43. doi: 10.1159/000409860. [DOI] [PubMed] [Google Scholar]
  21. Zeevi A., Scheffel C., Annen K., Duquesnoy R. J. Primed lymphocyte testing specificity of alloreactive lymphocyte clones for HLA-B locus determinants. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1440–1444. doi: 10.1073/pnas.80.5.1440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. el-Daher N., Keefer M. C., Reichman R. C., Dolin R., Roberts N. J., Jr Persisting human immunodeficiency virus type 1 gp160-specific human T lymphocyte responses including CD8+ cytotoxic activity after receipt of envelope vaccines. J Infect Dis. 1993 Aug;168(2):306–313. doi: 10.1093/infdis/168.2.306. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Diagnostic Laboratory Immunology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES