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. 1982 Jun;2(6):701–707. doi: 10.1128/mcb.2.6.701

Further Evidence That the Majority of Primary Nuclear RNA Transcripts in Mammalian Cells Do Not Contribute to mRNA

M Salditt-Georgieff 1, J E Darnell Jr 1
PMCID: PMC369846  PMID: 14582165

Abstract

Nuclear RNA from Chinese hamster ovary cells was effectively separated into polyadenylic acid [poly(A)]-containing [poly (A)+] and non-poly(A)-containing [poly(A)−] fractions so that ∼90% of the poly(A) was present in the (A)+ fraction. Only 25% of the 5′-terminal caps of the large nuclear molecules were present in the (A)+ class, but about 70% of the specific mRNA sequences (assayed with cDNA clones) were in the (A)+ class. It appears that many long capped heterogeneous nuclear RNA molecules are of a different sequence category from those molecules that are successfully processed into mRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berget S. M., Moore C., Sharp P. A. Spliced segments at the 5' terminus of adenovirus 2 late mRNA. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171–3175. doi: 10.1073/pnas.74.8.3171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chow L. T., Gelinas R. E., Broker T. R., Roberts R. J. An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA. Cell. 1977 Sep;12(1):1–8. doi: 10.1016/0092-8674(77)90180-5. [DOI] [PubMed] [Google Scholar]
  3. Darnell J. E., Jr Ribonucleic acids from animal cells. Bacteriol Rev. 1968 Sep;32(3):262–290. doi: 10.1128/br.32.3.262-290.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Darnell J. E., Jr Transcription units for mRNA production in eukaryotic cells and their DNA viruses. Prog Nucleic Acid Res Mol Biol. 1979;22:327–353. doi: 10.1016/s0079-6603(08)60803-x. [DOI] [PubMed] [Google Scholar]
  5. Derman E., Goldberg S., Darnell J. E., Jr hnRNA in HeLa cells: distribution of transcript sizes estimated from nascent molecule profile. Cell. 1976 Nov;9(3):465–472. doi: 10.1016/0092-8674(76)90091-x. [DOI] [PubMed] [Google Scholar]
  6. Evans R., Weber J., Ziff E., Darnell J. E. Premature termination during adenovirus transcription. Nature. 1979 Mar 22;278(5702):367–370. doi: 10.1038/278367a0. [DOI] [PubMed] [Google Scholar]
  7. Goldberg S., Schwartz H., Darnell J. E., Jr Evidence from UV transcription mapping in HeLa cells that heterogeneous nuclear RNA is the messenger RNA precursor. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4520–4523. doi: 10.1073/pnas.74.10.4520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harpold M. M., Evans R. M., Salditt-Georgieff M., Darnell J. E. Production of mRNA in Chinese hamster cells: relationship of the rate of synthesis to the cytoplasmic concentration of nine specific mRNA sequences. Cell. 1979 Aug;17(4):1025–1035. doi: 10.1016/0092-8674(79)90341-6. [DOI] [PubMed] [Google Scholar]
  9. Harpold M. M., Wilson M. C., Darnell J. E., Jr Chinese hamster polyadenylated messenger ribonucleic acid: relationship to non-polyadenylated sequences and relative conservation during messenger ribonucleic acid processing. Mol Cell Biol. 1981 Feb;1(2):188–198. doi: 10.1128/mcb.1.2.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Holland C. A., Mayrand S., Pederson T. Sequence complexity of nuclear and messenger RNA in HeLa cells. J Mol Biol. 1980 Apr 25;138(4):755–778. doi: 10.1016/0022-2836(80)90064-9. [DOI] [PubMed] [Google Scholar]
  11. Hough B. R., Smith M. J., Britten R. J., Davidson E. H. Sequence complexity of heterogeneous nuclear RNA in sea urchin embryos. Cell. 1975 Jul;5(3):291–299. doi: 10.1016/0092-8674(75)90104-x. [DOI] [PubMed] [Google Scholar]
  12. Klessig D. F. Two adenovirus mRNAs have a common 5' terminal leader sequence encoded at least 10 kb upstream from their main coding regions. Cell. 1977 Sep;12(1):9–21. doi: 10.1016/0092-8674(77)90181-7. [DOI] [PubMed] [Google Scholar]
  13. Lengyel J., Penman S. hnRNA size and processing as related to different DNA content in two dipterans: Drosophila and Aedes. Cell. 1975 Jul;5(3):281–290. doi: 10.1016/0092-8674(75)90103-8. [DOI] [PubMed] [Google Scholar]
  14. Maxwell I. H., Maxwell F., Hahn W. E. General occurrence and transcription of intervening sequences in mouse genes expressed via polyadenylated mRNA. Nucleic Acids Res. 1980 Dec 11;8(23):5875–5894. doi: 10.1093/nar/8.23.5875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nakazato H., Edmonds M. Purification of messenger RNA and heterogeneous nuclear RNA containing poly(a) sequences. Methods Enzymol. 1974;29:431–443. doi: 10.1016/0076-6879(74)29035-9. [DOI] [PubMed] [Google Scholar]
  16. Salditt-Georgieff M., Harpold M., Chen-Kiang S., Darnell J. E., Jr The addition of 5' cap structures occurs early in hnRNA synthesis and prematurely terminated molecules are capped. Cell. 1980 Jan;19(1):69–78. doi: 10.1016/0092-8674(80)90389-x. [DOI] [PubMed] [Google Scholar]
  17. Salditt-Georgieff M., Harpold M., Sawicki S., Nevins J., Darnell J. E., Jr Addition of poly(A) to nuclear RNA occurs soon after RNA synthesis. J Cell Biol. 1980 Sep;86(3):844–848. doi: 10.1083/jcb.86.3.844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Salditt-Georgieff M., Jelinek W., Darnell J. E., Furuichi Y., Morgan M., Shatkin A. Methyl labeling of HeLa cell hnRNA: a comparison with mRNA. Cell. 1976 Feb;7(2):227–237. doi: 10.1016/0092-8674(76)90022-2. [DOI] [PubMed] [Google Scholar]
  19. Sawicki S. G., Jelinek W., Darnell J. E. 3'-Terminal addition to HeLa cell nuclear and cytoplasmic poly (A). J Mol Biol. 1977 Jun 15;113(1):219–235. doi: 10.1016/0022-2836(77)90051-1. [DOI] [PubMed] [Google Scholar]
  20. Sehgal P. B., Derman E., Molloy G. R., Tamm I., Darnell J. E. 5,6-Dichloro-1-Beta-D-ribofuranosylbenzimidazole inhibits initiation of nuclear heterogeneous RNA chains in HeLa cells. Science. 1976 Oct 22;194(4263):431–433. doi: 10.1126/science.982026. [DOI] [PubMed] [Google Scholar]
  21. Sheiness D., Darnell J. E. Polyadenylic acid segment in mRNA becomes shorter with age. Nat New Biol. 1973 Feb 28;241(113):265–268. doi: 10.1038/newbio241265a0. [DOI] [PubMed] [Google Scholar]
  22. Soeiro R., Vaughan M. H., Warner J. R., Darnell J. E., Jr The turnover of nuclear DNA-like RNA in HeLa cells. J Cell Biol. 1968 Oct;39(1):112–118. doi: 10.1083/jcb.39.1.112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Venkatesan S., Nakazato H., Kopp D. W., Edmonds M. Properties of a small transcribed poly A sequence in heterogeneous nuclear RNA of HeLa cells. Nucleic Acids Res. 1979 Mar;6(3):1097–1110. doi: 10.1093/nar/6.3.1097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wagner A. F., Bugianesi R. L., Shen T. Y. Preparation of sepharose-bound poly (rI:rC). Biochem Biophys Res Commun. 1971 Oct 1;45(1):184–189. doi: 10.1016/0006-291x(71)90067-2. [DOI] [PubMed] [Google Scholar]

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