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. 1982 Jun;2(6):701–707. doi: 10.1128/mcb.2.6.701

Further Evidence That the Majority of Primary Nuclear RNA Transcripts in Mammalian Cells Do Not Contribute to mRNA

M Salditt-Georgieff 1, J E Darnell Jr 1
PMCID: PMC369846  PMID: 14582165

Abstract

Nuclear RNA from Chinese hamster ovary cells was effectively separated into polyadenylic acid [poly(A)]-containing [poly (A)+] and non-poly(A)-containing [poly(A)−] fractions so that ∼90% of the poly(A) was present in the (A)+ fraction. Only 25% of the 5′-terminal caps of the large nuclear molecules were present in the (A)+ class, but about 70% of the specific mRNA sequences (assayed with cDNA clones) were in the (A)+ class. It appears that many long capped heterogeneous nuclear RNA molecules are of a different sequence category from those molecules that are successfully processed into mRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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