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. 1977 Nov;60(5):1174–1182. doi: 10.1172/JCI108870

Glucagon release from rat pancreatic islets. A combined morphological and functional approach.

J L Carpentier, F Malaisse-Lagae, W A Müller
PMCID: PMC372471  PMID: 409734

Abstract

The release of glucagon induced in isolated rat islets by arginine or by calcium deprivation has been subjected to combined functional and morphological quantifications. Arginine-stimulated glucagon release was associated with a significant increase of morphological events linked to exocytosis. By contrast, the paradoxical events linked to exocytosis. By contrast, the paradoxical release of glucagon provoked by calcium deprivation, although accompanied by a significant loss of granule stores, was not associated with an increase of morphologically detectable exocytosis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chau-Wong M., Seeman P. The control of membrane-bound Ca 2+ by ATP. Biochim Biophys Acta. 1971 Aug 13;241(2):473–482. doi: 10.1016/0005-2736(71)90046-0. [DOI] [PubMed] [Google Scholar]
  2. Esterhuizen A. C., Howell S. L. Ultrastructure of the A-cells of cat islets of Langerhans following sympathetic stimulation of glucagon secretion. J Cell Biol. 1970 Sep;46(3):593–598. doi: 10.1083/jcb.46.3.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fujimoto W. Y., Ensinck J. W. Somatostatin inhibition of insulin and glucagon secretion in rat islet culture: reversal by ionophore A23187. Endocrinology. 1976 Jan;98(1):259–262. doi: 10.1210/endo-98-1-259. [DOI] [PubMed] [Google Scholar]
  4. Gerich J. E., Charles M. A., Grodsky G. M. Characterization of the effects of arginine and glucose on glucagon and insulin release from the perfused rat pancreas. J Clin Invest. 1974 Oct;54(4):833–841. doi: 10.1172/JCI107823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gerich J. E., Frankel B. J., Fanska R., West L., Forsham P. H., Grodsky G. M. Calcium dependency of glucagon secretion from the in vitro perfused rat pancreas. Endocrinology. 1974 May;94(5):1381–1385. doi: 10.1210/endo-94-5-1381. [DOI] [PubMed] [Google Scholar]
  6. Gomez-Acebo J., Parrilla R., Candela J. L. Fine structure of the A and D cells of the rabbit endocrine pancreas in vivo and incubated in vitro. I. Mechanism of secretion of the A cells. J Cell Biol. 1968 Jan;36(1):33–44. doi: 10.1083/jcb.36.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Herbert V., Lau K. S., Gottlieb C. W., Bleicher S. J. Coated charcoal immunoassay of insulin. J Clin Endocrinol Metab. 1965 Oct;25(10):1375–1384. doi: 10.1210/jcem-25-10-1375. [DOI] [PubMed] [Google Scholar]
  8. Lacy P. E., Kostianovsky M. Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes. 1967 Jan;16(1):35–39. doi: 10.2337/diab.16.1.35. [DOI] [PubMed] [Google Scholar]
  9. Lazarus S. S., Shapiro S., Volk B. W. Secretory granule formation and release in rabbit pancreatic A-cells. Diabetes. 1968 Mar;17(3):152–160. doi: 10.2337/diab.17.3.152. [DOI] [PubMed] [Google Scholar]
  10. Leclercq-Meyer V., Marchand J., Malaisse W. J. The role of calcium in glucagon release, interactions between glucose and calcium. Diabetologia. 1976 Oct;12(5):531–538. doi: 10.1007/BF01219520. [DOI] [PubMed] [Google Scholar]
  11. Leclercq-Meyer V., Rebelledo O., Marchand J., Malaisse W. Glucagon release: paradoxical stimulation by glucose during calcium deprivation. Science. 1975 Sep 12;189(4206):897–899. doi: 10.1126/science.1098149. [DOI] [PubMed] [Google Scholar]
  12. Lundquist I., Fanska R., Grodsky G. M. Interaction of calcium and glucose on glucagon secretion. Endocrinology. 1976 Nov;99(5):1304–1312. doi: 10.1210/endo-99-5-1304. [DOI] [PubMed] [Google Scholar]
  13. MUNGER B. L. The secretory cycle of the pancreatic islet alpha-cell. An electron microscopic study of normal and synthalin-treated rabbits. Lab Invest. 1962 Nov;11:885–901. [PubMed] [Google Scholar]
  14. Machino M., Onoe T., Sakuma H. Electron microscopic observations on the islet alpha cells of the domestic fowl pancreas. J Electron Microsc (Tokyo) 1966;15(4):249–256. [PubMed] [Google Scholar]
  15. Malaisse W., Malaisse-Lagae F., Wright P. H. A new method for the measurement in vitro of pancreatic insulin secretion. Endocrinology. 1967 Jan;80(1):99–108. doi: 10.1210/endo-80-1-99. [DOI] [PubMed] [Google Scholar]
  16. Orci L. A portrait of the pancreatic B-cell. The Minkowski Award Lecture delivered on July 19, 1973, during the 8th Congress of the International Diabetes Federation, held in Brussels, Belgium. Diabetologia. 1974 Jun;10(3):163–187. doi: 10.1007/BF00423031. [DOI] [PubMed] [Google Scholar]
  17. Orci L., Amherdt M., Malaisse-Lagae F., Rouiller C., Renold A. E. Insulin release by emiocytosis: demonstration with freeze-etching technique. Science. 1973 Jan 5;179(4068):82–84. doi: 10.1126/science.179.4068.82. [DOI] [PubMed] [Google Scholar]
  18. Orci L., Malaisse-Lagae F., Ravazzola M., Rouiller D., Renold A. E., Perrelet A., Unger R. A morphological basis for intercellular communication between alpha- and beta-cells in the endocrine pancreas. J Clin Invest. 1975 Oct;56(4):1066–1070. doi: 10.1172/JCI108154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pagliara A. S., Stillings S. N., Hover B., Martin D. M., Matschinsky F. M. Glucose modulation of amino acid-induced glucagon and insulin release in the isolated perfused rat pancreas. J Clin Invest. 1974 Oct;54(4):819–832. doi: 10.1172/JCI107822. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ravazzola M., Malaisse-Lagae F., Amherdt M., Perrelet A., Malaisse W. J., Orci L. Patterns of calcium localization in pancreatic endocrine cells. J Cell Sci. 1976 Jun;21(1):107–117. doi: 10.1242/jcs.21.1.107. [DOI] [PubMed] [Google Scholar]
  21. Rhoten W. B. The cellular response of saurian pancreatic islets to chronic insulin administration. Gen Comp Endocrinol. 1973 Jun;20(3):474–489. doi: 10.1016/0016-6480(73)90079-8. [DOI] [PubMed] [Google Scholar]
  22. Unger R. H. The Banting Memorial Lecture 1975. Diabetes and the alpha cell. Diabetes. 1976 Feb;25(2):136–151. doi: 10.2337/diab.25.2.136. [DOI] [PubMed] [Google Scholar]
  23. Weibel E. R. Stereological principles for morphometry in electron microscopic cytology. Int Rev Cytol. 1969;26:235–302. doi: 10.1016/s0074-7696(08)61637-x. [DOI] [PubMed] [Google Scholar]
  24. Wollheim C. B., Blondel B., Renold A. E., Sharp G. W. Calcium induced glucagon release in monolayer culture of the endocrine pancreas. Studies with ionophore A23187. Diabetologia. 1976 Aug;12(4):287–294. doi: 10.1007/BF00420970. [DOI] [PubMed] [Google Scholar]

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