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. 1969 Dec;18(6):1060–1064. doi: 10.1128/am.18.6.1060-1064.1969

Applicability of Aeration and Delayed Addition of Selenite to the Isolation of Salmonellae

John A Alford 1, Nancy L Knight 1
PMCID: PMC378193  PMID: 4905697

Abstract

The effect of aeration combined with the delayed addition of selenite on the lag period of several strains of salmonellae and other enterobacteria is reported. A procedure has been developed involving shaking of the sample in a basal medium for 4 hr at 37 C, adding selenite and cystine, and continuing shaking for 20 hr. Confirmation by selective plating, biochemical tests, and serology gave results comparable to the standard lactose pre-enrichment method with the saving of 24 hr and elimination of one set of media. Confirmation by fluorescent-antibody tests showed that fewer positive fluorescent stains were obtained from the aerated procedure than from the lactose pre-enrichment procedure. Precautions in the application of this procedure are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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