Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1969 Dec;18(6):1060–1064. doi: 10.1128/am.18.6.1060-1064.1969

Applicability of Aeration and Delayed Addition of Selenite to the Isolation of Salmonellae

John A Alford 1, Nancy L Knight 1
PMCID: PMC378193  PMID: 4905697

Abstract

The effect of aeration combined with the delayed addition of selenite on the lag period of several strains of salmonellae and other enterobacteria is reported. A procedure has been developed involving shaking of the sample in a basal medium for 4 hr at 37 C, adding selenite and cystine, and continuing shaking for 20 hr. Confirmation by selective plating, biochemical tests, and serology gave results comparable to the standard lactose pre-enrichment method with the saving of 24 hr and elimination of one set of media. Confirmation by fluorescent-antibody tests showed that fewer positive fluorescent stains were obtained from the aerated procedure than from the lactose pre-enrichment procedure. Precautions in the application of this procedure are discussed.

Full text

PDF
1063

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Busta F. F., Speck M. L. Antimicrobial effect of cocoa on salmonellae. Appl Microbiol. 1968 Feb;16(2):424–425. doi: 10.1128/am.16.2.424-425.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HAGLUND J. R., AYRES J. C., PATON A. M., KRAFT A. A., QUINN L. Y. DETECTION OF SALMONELLA IN EGGS AND EGG PRODUCTS WITH FLUORESCENT ANTIBODY. Appl Microbiol. 1964 Sep;12:447–450. doi: 10.1128/am.12.5.447-450.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Iandolo J. J., Ordal Z. J. Repair of thermal injury of Staphylococcus aureus. J Bacteriol. 1966 Jan;91(1):134–142. doi: 10.1128/jb.91.1.134-142.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Insalata N. F., Schulte S. J., Berman J. H. Immunofluorescence technique for the detection of salmonellae in various foods. Appl Microbiol. 1967 Sep;15(5):1145–1149. doi: 10.1128/am.15.5.1145-1149.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. King S., Metzger W. I. A new plating medium for the isolation of enteric pathogens. I. hektoen enteric agar. Appl Microbiol. 1968 Apr;16(4):577–578. doi: 10.1128/am.16.4.577-578.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Raj H. Enrichment medium for selection of Salmonella from fish homogenate. Appl Microbiol. 1966 Jan;14(1):12–20. doi: 10.1128/am.14.1.12-20.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. SILLIKER J. H., DEIBEL R. H., CHIU J. Y. OCCURRENCE OF GRAM-POSITIVE ORGANISMS POSSESSING CHARACTERISTICS SIMILAR TO THOSE OF SALMONELLA AND THE PRACTICAL PROBLEM OF RAPID AND DEFINITIVE SALMONELLA IDENTIFICATION. Appl Microbiol. 1964 Sep;12:395–399. doi: 10.1128/am.12.5.395-399.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sogin S. J., Ordal Z. J. Regeneration of ribosomes and ribosomal ribonucleic acid during repair of thermal injury to Staphylococcus. J Bacteriol. 1967 Oct;94(4):1082–1087. doi: 10.1128/jb.94.4.1082-1087.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sperber W. H., Deibel R. H. Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions. Appl Microbiol. 1969 Apr;17(4):533–539. doi: 10.1128/am.17.4.533-539.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Weiss K. F., Ayres J. C., Kraft A. A. Inhibitory action of selenite on Escherichia coli, Proteus vulgaris, and Salmonella thompson. J Bacteriol. 1965 Oct;90(4):857–862. doi: 10.1128/jb.90.4.857-862.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES