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. 1974 Jan;27(1):224–231. doi: 10.1128/am.27.1.224-231.1974

Adaptation and Infection of Mouse Bone Marrow (JLS-V9) Cells in Suspension Culture for Production of Rauscher Leukemia Virus

Howard M Hodge 1, Frederick Klein 1, Alok K Bandyopadhyay 1, Orson R Robinson Jr 1, George P Shibley 1
PMCID: PMC379998  PMID: 4129475

Abstract

JLS-V9 mouse bone marrow cells were readily adapted to suspension culture, chronically infected with Rauscher leukemia virus (RLV), and subsequently grown in 7.5- and 14-liter New Brunswick fermentors. The suspension-type cell system can be modified to produce virus with clearly defined properties, such as high ribonucleic acid-dependent deoxyribonucleic acid polymerase (RDDP) activity, high particle count, and high infectious particle count. Biological and biophysical properties of suspension-produced RLV were not affected by concentration and purification employing continuous-flow and rate-zonal centrifugation procedures. The RDDP assay was standardized and showed a linear incorporation of 3H-thymidine 5′-monophosphate (3H-TMP) up to 30 min. Further characterization indicated that a high percentage of 3H-TMP incorporation was due to RDDP.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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