Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1972 Jan;23(1):130–134. doi: 10.1128/am.23.1.130-134.1972

Reproducible Topical Staphylococcal Infection in Rats1

Faith Goldschmidt a
PMCID: PMC380290  PMID: 5059616

Abstract

A topical infection model for the study of the effectiveness of antimicrobials was developed. Animals were laparotomized, sutured with braided silk, and inoculated with a strain of Staphylococcus aureus. The test organism was phage typed, and its antibiotic spectrum was determined. Concentrations of bacteria from 5 × 104 to 108 cells per incision produced large body wall stitch abscesses with occasional drainage through the skin. This laparotomy infection is readily reproducible and can be used for evaluation of the ability of topical antimicrobials to prevent S. aureus stitch abscesses.

Full text

PDF
131

Images in this article

131Image
on p.131
131Image
on p.131
131Image
on p.131
131Image
on p.131

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALAMI S. Y., KELLY F. C. Influence of coagulases and route of injection on staphylococcal virulence in mice. Proc Soc Exp Biol Med. 1960 Dec;105:589–591. doi: 10.3181/00379727-105-26186. [DOI] [PubMed] [Google Scholar]
  2. Agarwal D. S. Subcutaneous staphylococcal infection in mice. 3. Effect of active and passive immunization and anti-inflammatory drugs. Br J Exp Pathol. 1967 Oct;48(5):483–500. [PMC free article] [PubMed] [Google Scholar]
  3. Agarwal D. S. Subcutaneous staphylococcal infection in mice. I. The role of cotton-dust in enhancing infection. Br J Exp Pathol. 1967 Aug;48(4):436–449. [PMC free article] [PubMed] [Google Scholar]
  4. Agarwal D. S. Subcutaneous staphylococcal infection in mice. II. The inflammatory response to different strains of staphylococci and micrococci. Br J Exp Pathol. 1967 Oct;48(5):468–482. [PMC free article] [PubMed] [Google Scholar]
  5. BURNETT J. W. TRAUMA IN EXPERIMENTAL SUPERFICIAL CUTANEOUS INFECTIONS. Arch Dermatol. 1963 Sep;88:276–279. doi: 10.1001/archderm.1963.01590210034005. [DOI] [PubMed] [Google Scholar]
  6. Duncan W. C., McBride M. E., Knox J. M. Experimental production of infections in humans. J Invest Dermatol. 1970 Apr;54(4):319–323. doi: 10.1111/1523-1747.ep12258627. [DOI] [PubMed] [Google Scholar]
  7. Edlich R. F., Custer J., Madden J., Dajani A. S., Rogers W., Wangensteen O. H. Studies in management of the contaminated wound. 3. Assessment of the effectiveness of irrigation with antiseptic agents. Am J Surg. 1969 Jul;118(1):21–30. doi: 10.1016/0002-9610(69)90089-0. [DOI] [PubMed] [Google Scholar]
  8. Edlich R. F., Rogers W., Kasper G., Kaufman D., Tsung M. S., Wangensteen O. H. Studies in the management of the contaminated wound. I. Optimal time for closure of contaminated open wounds. II. Comparison of resistance to infection of open and closed wounds during healing. Am J Surg. 1969 Mar;117(3):323–329. doi: 10.1016/0002-9610(69)90365-1. [DOI] [PubMed] [Google Scholar]
  9. Edlich R. F., Tsung M. S., Rogers W., Rogers P., Wangensteen O. H. Studies in management of the contaminated wound. I. Technique of closure of such wounds together with a note on a reproducible experimental model. J Surg Res. 1968 Dec;8(12):585–592. doi: 10.1016/0022-4804(68)90013-9. [DOI] [PubMed] [Google Scholar]
  10. Glotzer D. J., Goodman W. S., Geronimus L. H. Topical antibiotic prophylaxis in contaminated wounds. Experimental evaluation. Arch Surg. 1970 May;100(5):589–593. doi: 10.1001/archsurg.1970.01340230055012. [DOI] [PubMed] [Google Scholar]
  11. Hunt T. K., Jawetz E., Hutchison J. G., Dunphy J. E. A new model for the study of wound infection. J Trauma. 1967 Mar;7(2):298–306. doi: 10.1097/00005373-196703000-00012. [DOI] [PubMed] [Google Scholar]
  12. JAMES R. C., MACLEOD C. J. Induction of staphylococcal infections in mice with small inocula introduced on sutures. Br J Exp Pathol. 1961 Jun;42:266–277. [PMC free article] [PubMed] [Google Scholar]
  13. Miraglia G. J. Model infections in the evaluation of antimicrobial agents. Trans N Y Acad Sci. 1970 Mar;32(3):337–347. doi: 10.1111/j.2164-0947.1970.tb02065.x. [DOI] [PubMed] [Google Scholar]
  14. Möller A., Rydberg B. influence of a cationic detergent on the development of infection in experimental wounds contaminated with staphylococci. Acta Chir Scand. 1969;135(6):459–465. [PubMed] [Google Scholar]
  15. Noble W. C. The production of subcutaneous staphylococcal skin lesions in mice. Br J Exp Pathol. 1965 Jun;46(3):254–262. [PMC free article] [PubMed] [Google Scholar]
  16. Russell H. E., Gutekunst D. P., Chamberlain R. E. Evaluation of furazolium chloride in topical treatment of model infections in laboratory animals. Antimicrob Agents Chemother (Bethesda) 1967;7:497–501. [PubMed] [Google Scholar]
  17. Smith M., Enquist I. F. A quantitative study of impaired healing resulting from infection. Surg Gynecol Obstet. 1967 Nov;125(5):965–973. [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES