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. 1972 May;23(5):841–845. doi: 10.1128/am.23.5.841-845.1972

Preparation of Purified Suspensions of Coxiella burneti by Genetron Extraction Followed by Continuous-Flow Ultracentrifugation1

Doria R Dubois 1,2, Ernest C Cutchins 1,2, Sanford Berman 1,2, Joseph P Lowenthal 1,2, Robert L Timchak 1,2
PMCID: PMC380458  PMID: 4555634

Abstract

A method for the preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow density gradient ultracentrifugation is described. Both phases of the Henzerling strain of C. burneti were found in a zone between 53 and 65% (w/w) sucrose. Based on chemical assays, the Genetron zonal rickettsial suspensions were found to be as pure as the rickettsial suspensions which were prepared by the ether extraction method currently in use for producing Q fever vaccines for human use.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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