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. 1973 Apr;25(4):584–588. doi: 10.1128/am.25.4.584-588.1973

Production and Purification of Acid Protease from the Thermophilic Fungus, Penicillium duponti K1014

Hikotaka Hashimoto 1, Yukio Kaneko 1, Takashi Iwaasa 1, Tamotsu Yokotsuka 1
PMCID: PMC380866  PMID: 4699218

Abstract

A thermophilic fungus, Penicillium duponti K1014, produced an acid protease in a submerged culture. Maximum enzyme production, in a 30-liter fermentor at a preselected optimum growth temperature of 50 C, occurred after 3 days. The productivity of the enzyme was associated with changes of viscosity and pH of culture broth during the growth. The acid protease was isolated from the culture filtrates and was purified ninefold by alcohol precipitation, column chromatography on O-(diethylaminoethyl)cellulose, batchwise treatment with O-(carboxymethyl)cellulose, and by gel filtration. The purified enzyme was homogeneous on disk electrophoresis and sedimentation analysis. The sedimentation constant s20.w, calculated at a concentration of 0.64%, was 3.98S.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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