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. 1979 Mar;76(3):1256–1260. doi: 10.1073/pnas.76.3.1256

Differentiation of a rat mammary cell line in vitro.

R Dulbecco, M Bologna, M Unger
PMCID: PMC383229  PMID: 375233

Abstract

We have studied the development of fusiform (probably related to myoepithelial) cells in Rama 25 cultures [Bennett, D. C. Peachey, L. A., Durbin, H. & Rudland, P. S. (1978) Cell 15, 283--298]; we show that they are generated from special differentiated structures (projections) that contain a rapidly differentiating cell type (F-precursor cells). Clonal sublines isolated from projections develop in several directions under both environmental and genetic control. Some types of differentiation are reversible; others are irreversible. The various cell occurring in vitro may correspond to specific cell types in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett D. C., Peachey L. A., Durbin H., Rudland P. S. A possible mammary stem cell line. Cell. 1978 Sep;15(1):283–298. doi: 10.1016/0092-8674(78)90104-6. [DOI] [PubMed] [Google Scholar]
  2. Das N. K., Hosick H. L., Nandi S. Influence of seeding density on multicellular organization and nuclear events in cultures of normal and neoplastic mouse mammary epithelium. J Natl Cancer Inst. 1974 Mar;52(3):849–861. doi: 10.1093/jnci/52.3.849. [DOI] [PubMed] [Google Scholar]
  3. EBNER K. E., HOOVER C. R., HAGEMAN E. C., LARSON B. L. Cultivation and properties of bovine mammary cell cultures. Exp Cell Res. 1961 Mar;23:373–385. doi: 10.1016/0014-4827(61)90046-5. [DOI] [PubMed] [Google Scholar]
  4. Gröschel-Stewart U., Ceurremans S., Lehr I., Mahlmeister C., Paar E. Production of specific antibodies to contractile proteins, and their use in immunofluorescence microscopy. II. Species-specific and species-non-specific antibodies to smooth and striated chicken muscle actin. Histochemistry. 1977 Feb 1;50(4):271–279. doi: 10.1007/BF00507120. [DOI] [PubMed] [Google Scholar]
  5. Haslam S. Z., Bern H. A. Histopathogenesis of 7,12-diemthylbenz(a)anthracene-induced rat mammary tumors. Proc Natl Acad Sci U S A. 1977 Sep;74(9):4020–4024. doi: 10.1073/pnas.74.9.4020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LASFARGUES E. Y. Cultivation and behavior in vitro of the normal mammary epithelium of the adult mouse. Anat Rec. 1957 Jan;127(1):117–129. doi: 10.1002/ar.1091270111. [DOI] [PubMed] [Google Scholar]
  7. Lennon V. A., Unger M., Dulbecco R. Thy-1: a differentiation marker of potential mammary myoepithelial cells in vitro. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6093–6097. doi: 10.1073/pnas.75.12.6093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Linzell J. L., Peaker M. Mechanism of milk secretion. Physiol Rev. 1971 Jul;51(3):564–597. doi: 10.1152/physrev.1971.51.3.564. [DOI] [PubMed] [Google Scholar]
  9. Linzell J. L. The effect of very frequent milking and of oxytocin on the yield and composition of milk in fed and fasted goats. J Physiol. 1967 May;190(2):333–346. doi: 10.1113/jphysiol.1967.sp008212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MACKENZIE D. D., LASCELLES A. K. THE VARIATION IN THE COMPOSITION OF MILK DURING SUCCESSIVE STAGES OF MILK REMOVAL FROM THE MAMMARY GLAND OF THE COW. Aust J Biol Sci. 1965 Apr;18:379–386. doi: 10.1071/bi9650379. [DOI] [PubMed] [Google Scholar]
  11. McGrath C. M., Nandi S., Young L. Relationship between organization of mammary tumors and the ability of tumor cells to replicate mammary tumor virus and to recognize growth-inhibitory contact signals in vitro. J Virol. 1972 Feb;9(2):367–376. doi: 10.1128/jvi.9.2.367-376.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McGrath C. M. Replication of mammary tumor virus in tumor cell cultures: dependence on hormone-induced cellular organization. J Natl Cancer Inst. 1971 Aug;47(2):455–467. [PubMed] [Google Scholar]
  13. Misfeldt D. S., Hamamoto S. T., Pitelka D. R. Transepithelial transport in cell culture. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1212–1216. doi: 10.1073/pnas.73.4.1212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Owens R. B., Hackett A. J. Tissue culture studies of mouse mammary tumor cells and associated viruses. J Natl Cancer Inst. 1972 Nov;49(5):1321–1332. [PubMed] [Google Scholar]
  15. Parks W. P., Scolnick E. M. Murine mammary tumor cell clones with varying degrees of virus expression. Virology. 1973 Sep;55(1):163–173. doi: 10.1016/s0042-6822(73)81018-9. [DOI] [PubMed] [Google Scholar]
  16. Qasba P. K., Gullino P. M. alpha-Lactalbumin content of rat mammary carcinomas and the effect of pituitary stimulation. Cancer Res. 1977 Oct;37(10):3792–3795. [PubMed] [Google Scholar]
  17. Schultz G. S., Ebner K. E. Measurement of alpha-lactalbumin in serum and mammary tumors of rats by radioimmunoassay. Cancer Res. 1977 Dec;37(12):4482–4488. [PubMed] [Google Scholar]
  18. Sykes J. A., Whitescarver J., Briggs L. Observations on a cell line producing mammary tumor virus. J Natl Cancer Inst. 1968 Dec;41(6):1315–1327. [PubMed] [Google Scholar]
  19. Visser A. S., Prop F. J. "Domes," periodically expanding and collapsing secretory structures in cell cultures of mouse mammary tumors. J Natl Cancer Inst. 1974 Jan;52(1):293–295. doi: 10.1093/jnci/52.1.293. [DOI] [PubMed] [Google Scholar]
  20. Yagi M. J. Cultivation and characterization of BALB-cfC3H mammary tumor cell lines. J Natl Cancer Inst. 1973 Dec;51(6):1849–1860. doi: 10.1093/jnci/51.6.1849. [DOI] [PubMed] [Google Scholar]
  21. Zaks M. G., Natochin Iu V., Sokolova N. N., Tanasiichuk O. F., Tverskoi G. B. O transporte natriia i kaliia pri obrazovanii moloka. Fiziol Zh SSSR Im I M Sechenova. 1965 Apr;51(4):513–519. [PubMed] [Google Scholar]

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