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. 1979 Aug;76(8):3703–3707. doi: 10.1073/pnas.76.8.3703

Production and characterization of a monoclonal antibody to chicken type I collagen.

T F Linsenmayer, M J Hendrix, C D Little
PMCID: PMC383901  PMID: 291035

Abstract

We have shown that lymphocyte-myeloma cell hybridization can be used to produce large amounts of extremely high-titer specific antibodies against type I collagen, a macromolecule normally of low immunogenicity. In a passive hemagglutination assay the antibody had a high titer against chicken type I collagen but showed no activity against chicken type II or rat type I collagen. By using a two-step fluorescence histochemical procedure on sections of embryonic chicken tibia, strong fluorescence was observed in the perichondrium and surrounding connective tissue (known to contain type I collagen) but not over the cartilage (characterized by type II collagen). When used in conjunction with Staphylococcus aureus as a solid phase immunoadsorbant, the antibody was shown to bind to labeled collagen synthesized in vitro by embryonic chicken calvaria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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