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. 1974 Jun;71(6):2273–2275. doi: 10.1073/pnas.71.6.2273

In Vitro Repair of Apurinic Sites in DNA

W G Verly 1, F Gossard 1, P Crine 1
PMCID: PMC388434  PMID: 4601584

Abstract

Apurinic sites disappear from DNA during an incubation of the DNA with the Escherichia coli endonuclease specific for apurinic sites, DNA polymerase I (EC 2.7.7.7.), and T4 ligase (EC 6.5.1.1). Omission of any one of these three enzymes and, in particular, omission of the endonuclease specific for apurinic sites prevents this in vitro repair.

Keywords: DNA depurination, endonuclease specific for apurinic sites, DNA repair

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. APOSHIAN H. V., KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme. J Biol Chem. 1962 Feb;237:519–525. [PubMed] [Google Scholar]
  2. Brakier L., Verly W. G. The lethal action of ehtyl methanesulfonate, nitrogen mustard and myleran on the T7 coliphage. Biochim Biophys Acta. 1970 Aug 8;213(2):296–311. doi: 10.1016/0005-2787(70)90038-9. [DOI] [PubMed] [Google Scholar]
  3. Gillespie C. J., Gislason G. S., Dugle D. L., Chapman J. D. Random break analysis of DNA sedimentation profiles. Radiat Res. 1972 Aug;51(2):272–279. [PubMed] [Google Scholar]
  4. LAWLEY P. D., BROOKES P. FURTHER STUDIES ON THE ALKYLATION OF NUCLEIC ACIDS AND THEIR CONSTITUENT NUCLEOTIDES. Biochem J. 1963 Oct;89:127–138. doi: 10.1042/bj0890127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lawley P. D., Lethbridge J. H., Edwards P. A., Shooter K. V. Inactivation of bacteriophage T7 by mono- and difunctional sulphur mustards in relation to cross-linking and depurination of bacteriophage DNA. J Mol Biol. 1969 Jan 14;39(1):181–198. doi: 10.1016/0022-2836(69)90341-6. [DOI] [PubMed] [Google Scholar]
  6. Lindahl T., Nyberg B. Rate of depurination of native deoxyribonucleic acid. Biochemistry. 1972 Sep 12;11(19):3610–3618. doi: 10.1021/bi00769a018. [DOI] [PubMed] [Google Scholar]
  7. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  8. Paquette Y., Crine P., Verly W. G. Properties of the endonuclease for depurinated DNA from Escherichia coli. Can J Biochem. 1972 Nov;50(11):1199–1209. doi: 10.1139/o72-163. [DOI] [PubMed] [Google Scholar]
  9. RICHARDSON C. C., SCHILDKRAUT C. L., APOSHIAN H. V., KORNBERG A. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. J Biol Chem. 1964 Jan;239:222–232. [PubMed] [Google Scholar]
  10. SCHUSTER H. [The method of reaction of desoxyribonucleic acid with nitrous acid]. Z Naturforsch B. 1960 May;15B:298–304. [PubMed] [Google Scholar]
  11. Strauss B. S., Robbins M. DNA methylated in vitro by a monofunctional alkylating agent as a substrate for a specific nuclease from Micrococcus lysodeikticus. Biochim Biophys Acta. 1968 Jun 18;161(1):68–75. doi: 10.1016/0005-2787(68)90295-5. [DOI] [PubMed] [Google Scholar]
  12. Studier F. W. The genetics and physiology of bacteriophage T7. Virology. 1969 Nov;39(3):562–574. doi: 10.1016/0042-6822(69)90104-4. [DOI] [PubMed] [Google Scholar]
  13. TAMM C., SHAPIRO H. S., LIPSHITZ R., CHARGAFF E. Distribution density of nucleotides within a desoxyribonucleic acid chain. J Biol Chem. 1953 Aug;203(2):673–688. [PubMed] [Google Scholar]
  14. Verly W. G., Paquette Y. An endonuclease for depurinated DNA in Escherichia coli B. Can J Biochem. 1972 Feb;50(2):217–224. doi: 10.1139/o72-029. [DOI] [PubMed] [Google Scholar]
  15. Verly W. G., Paquette Y. An endonuclease for depurinated DNA in rat liver. Can J Biochem. 1973 Jul;51(7):1003–1009. doi: 10.1139/o73-130. [DOI] [PubMed] [Google Scholar]

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