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. 1972 Dec;69(12):3779–3783. doi: 10.1073/pnas.69.12.3779

Altered Chloroplast Ribosomal Proteins Associated with Erythromycin-Resistant Mutants in Two Genetic Systems of Chlamydomonas reinhardi

Laurens Mets 1,*, Lawrence Bogorad 1
PMCID: PMC389872  PMID: 4509340

Abstract

The phenotype of several erythromycin-resistant mutants of Chlamydomonas reinhardi was further characterized in terms of the electrophoretic properties of their chloroplast ribosomal proteins. In mutant ery-M2d a single protein of the large (52 S) subunit has altered properties, which probably result from a change in its primary sequence. This mutation is inherited in a Meudelian manner. In mutant ery-U1a, which is inherited in a uniparental manner, a different single protein of the 52 S subunit is altered. This change might result from a change in either the primary sequence of the protein or in some form of secondary modification. These results indicate that these two distinct genetic systems must cooperate in the production of chloroplast ribosomes.

Keywords: electrophoresis, Mendelian and uniparental inheritance, sucrose density centrifugation

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Selected References

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