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. 1985 Oct;82(20):6899–6902. doi: 10.1073/pnas.82.20.6899

Identification of a high molecular weight polypeptide that may be part of the circadian clockwork in Acetabularia

R Hartwig *, M Schweiger †, R Schweiger *, H G Schweiger *
PMCID: PMC390795  PMID: 16593618

Abstract

In the chloroplast fraction of the unicellular and uninucleate green alga Acetabularia, we have detected a Mr ≈230,000 protein (p230) whose synthesis exhibits a pronounced endogenous diurnal rhythm. As judged by scanning densitometry of fluorographs of NaDodSO4/polyacrylamide gels, the synthesis of other proteins in the same fraction was independent of the time in the cycle. The incorporation of [35S]methionine into p230 was completely inhibited by cycloheximide, whereas chloramphenicol had no effect. This strongly suggests that p230 is translated on 80S ribosomes. Eighthour periods of exposure to cycloheximide produced a shift in the phase of the oscillation of p230 synthesis. The results are consistent with the hypothesis that p230 is essential for expression of circadian rhythms in Acetabularia.

Keywords: biological clock, translation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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