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. 1984 Aug;81(15):4776–4780. doi: 10.1073/pnas.81.15.4776

Identification of a cloned cytokinin biosynthetic gene

G F Barry 1, S G Rogers 1, R T Fraley 1, L Brand 1
PMCID: PMC391573  PMID: 16593495

Abstract

A small region of the Ti plasmid (the tmr locus), thought to be involved in phytohormone metabolism in Agrobacterium tumefaciens-transformed plant tissue, was cloned and expressed in Escherichia coli. By enzyme assay, the tmr locus was shown to encode isopentenyltransferase, an enzyme that catalyzes the first step in cytokinin biosynthesis.

Keywords: Agrobacterium tumefaciens, Ti plasmid, tmr locus, isopentenyltransferase, phytohormone biosynthesis

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Selected References

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  1. Akiyoshi D. E., Morris R. O., Hinz R., Mischke B. S., Kosuge T., Garfinkel D. J., Gordon M. P., Nester E. W. Cytokinin/auxin balance in crown gall tumors is regulated by specific loci in the T-DNA. Proc Natl Acad Sci U S A. 1983 Jan;80(2):407–411. doi: 10.1073/pnas.80.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bartz J. K., Kline L. K., Söll D. N6-(Delta 2-isopentenyl)adenosine: biosynthesis in vitro in transfer RNA by an enzyme purified from Escherichia coli. Biochem Biophys Res Commun. 1970 Sep 30;40(6):1481–1487. doi: 10.1016/0006-291x(70)90035-5. [DOI] [PubMed] [Google Scholar]
  4. Bevan M. W., Chilton M. D. Multiple transcripts of T-DNA detected in nopaline crown gall tumors. J Mol Appl Genet. 1982;1(6):539–546. [PubMed] [Google Scholar]
  5. Braun A. C. A Physiological Basis for Autonomous Growth of the Crown-Gall Tumor Cell. Proc Natl Acad Sci U S A. 1958 Apr;44(4):344–349. doi: 10.1073/pnas.44.4.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chen C. M., Melitz D. K. Cytokinin biosynthesis in a cell-free system from cytokinin-autotrophic tobacco tissue cultures. FEBS Lett. 1979 Nov 1;107(1):15–20. doi: 10.1016/0014-5793(79)80452-4. [DOI] [PubMed] [Google Scholar]
  7. Chilton M. D., Drummond M. H., Merio D. J., Sciaky D., Montoya A. L., Gordon M. P., Nester E. W. Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell. 1977 Jun;11(2):263–271. doi: 10.1016/0092-8674(77)90043-5. [DOI] [PubMed] [Google Scholar]
  8. Garfinkel D. J., Simpson R. B., Ream L. W., White F. F., Gordon M. P., Nester E. W. Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis. Cell. 1981 Nov;27(1 Pt 2):143–153. doi: 10.1016/0092-8674(81)90368-8. [DOI] [PubMed] [Google Scholar]
  9. Gray J. E., Patin D. W., Calhoun D. H. Identification of the protein products of the rrnC, ilv, rho region of the Escherichia coli K-12 chromosome. Mol Gen Genet. 1981;183(3):428–436. doi: 10.1007/BF00268761. [DOI] [PubMed] [Google Scholar]
  10. Heidekamp F., Dirkse W. G., Hille J., van Ormondt H. Nucleotide sequence of the Agrobacterium tumefaciens octopine Ti plasmid-encoded tmr gene. Nucleic Acids Res. 1983 Sep 24;11(18):6211–6223. doi: 10.1093/nar/11.18.6211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Joos H., Inzé D., Caplan A., Sormann M., Van Montagu M., Schell J. Genetic analysis of T-DNA transcripts in nopaline crown galls. Cell. 1983 Apr;32(4):1057–1067. doi: 10.1016/0092-8674(83)90290-8. [DOI] [PubMed] [Google Scholar]
  12. Leemans J., Deblaere R., Willmitzer L., De Greve H., Hernalsteens J. P., Van Montagu M., Schell J. Genetic Identification of functions of TL-DNA transcripts in octopine crown galls. EMBO J. 1982;1(1):147–152. doi: 10.1002/j.1460-2075.1982.tb01138.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McCloskey J. A., Hashizume T., Basile B., Ohno Y., Sonoki S. Occurrence and levels of cis-and trans-zeatin ribosides in the culture medium of a virulent strain of Agrobacterium tumefaciens. FEBS Lett. 1980 Feb 25;111(1):181–183. doi: 10.1016/0014-5793(80)80788-5. [DOI] [PubMed] [Google Scholar]
  14. Nester E. W., Kosuge T. Plasmids specifying plant hyperplasias. Annu Rev Microbiol. 1981;35:531–565. doi: 10.1146/annurev.mi.35.100181.002531. [DOI] [PubMed] [Google Scholar]
  15. Rao R. N., Rogers S. G. Plasmid pKC7: a vector containing ten restriction endonuclease sites suitable for cloning DNA segments. Gene. 1979 Sep;7(1):79–82. doi: 10.1016/0378-1119(79)90044-1. [DOI] [PubMed] [Google Scholar]
  16. Regier D. A., Morris R. O. Secretion of trans-zeatin by Agrobacterium tumefaciens: a function determined by the nopaline Ti plasmid. Biochem Biophys Res Commun. 1982 Feb 26;104(4):1560–1566. doi: 10.1016/0006-291x(82)91429-2. [DOI] [PubMed] [Google Scholar]
  17. Rosenbaum N., Gefter M. L. Delta 2 -isopentenylpyrophosphate: transfer ribonucleic acid 2 -isopentenyltransferase from Escherichia coli. Purification and properties of the enzyme. J Biol Chem. 1972 Sep 25;247(18):5675–5680. [PubMed] [Google Scholar]
  18. Sancar A., Hack A. M., Rupp W. D. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. doi: 10.1128/jb.137.1.692-693.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schell J., Van Montagu M., De Beuckeleer M., De Block M., Depicker A., De Wilde M., Engler G., Genetello C., Hernalsteens J. P., Holsters M. Interactions and DNA transfer between Agrobacterium tumefaciens, the Ti-plasmid and the plant host. Proc R Soc Lond B Biol Sci. 1979 Apr 11;204(1155):251–266. doi: 10.1098/rspb.1979.0026. [DOI] [PubMed] [Google Scholar]
  20. Schröder G., Klipp W., Hillebrand A., Ehring R., Koncz C., Schröder J. The conserved part of the T-region in Ti-plasmids expresses four proteins in bacteria. EMBO J. 1983;2(3):403–409. doi: 10.1002/j.1460-2075.1983.tb01437.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Seeburg P. H., Sias S., Adelman J., de Boer H. A., Hayflick J., Jhurani P., Goeddel D. V., Heyneker H. L. Efficient bacterial expression of bovine and porcine growth hormones. DNA. 1983;2(1):37–45. doi: 10.1089/dna.1.1983.2.37. [DOI] [PubMed] [Google Scholar]
  22. Sutcliffe J. G. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737–3741. doi: 10.1073/pnas.75.8.3737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weinstock G. M., McEntee K., Lehman I. R. Hydrolysis of nucleoside triphosphates catalyzed by the recA protein of Escherichia coli. Characterization of ATP hydrolysis. J Biol Chem. 1981 Aug 25;256(16):8829–8834. [PubMed] [Google Scholar]
  24. Willmitzer L., Dhaese P., Schreier P. H., Schmalenbach W., Van Montagu M., Schell J. Size, location and polarity of T-DNA-encoded transcripts in nopaline crown gall tumors; common transcripts in octopine and nopaline tumors. Cell. 1983 Apr;32(4):1045–1056. doi: 10.1016/0092-8674(83)90289-1. [DOI] [PubMed] [Google Scholar]
  25. Willmitzer L., Simons G., Schell J. The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts. EMBO J. 1982;1(1):139–146. doi: 10.1002/j.1460-2075.1982.tb01137.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Zaenen I., Van Larebeke N., Van Montagu M., Schell J. Supercoiled circular DNA in crown-gall inducing Agrobacterium strains. J Mol Biol. 1974 Jun 15;86(1):109–127. doi: 10.1016/s0022-2836(74)80011-2. [DOI] [PubMed] [Google Scholar]

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