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. 1984 Aug;81(15):4776–4780. doi: 10.1073/pnas.81.15.4776

Identification of a cloned cytokinin biosynthetic gene

G F Barry 1, S G Rogers 1, R T Fraley 1, L Brand 1
PMCID: PMC391573  PMID: 16593495

Abstract

A small region of the Ti plasmid (the tmr locus), thought to be involved in phytohormone metabolism in Agrobacterium tumefaciens-transformed plant tissue, was cloned and expressed in Escherichia coli. By enzyme assay, the tmr locus was shown to encode isopentenyltransferase, an enzyme that catalyzes the first step in cytokinin biosynthesis.

Keywords: Agrobacterium tumefaciens, Ti plasmid, tmr locus, isopentenyltransferase, phytohormone biosynthesis

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Selected References

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