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. 1983 Jun;80(12):3758–3761. doi: 10.1073/pnas.80.12.3758

Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.

D Owerbach, A Lernmark, L Rask, P A Peterson, P Platz, A Svejgaard
PMCID: PMC394130  PMID: 6304735

Abstract

Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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