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. 1983 Jun;80(12):3758–3761. doi: 10.1073/pnas.80.12.3758

Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.

D Owerbach, A Lernmark, L Rask, P A Peterson, P Platz, A Svejgaard
PMCID: PMC394130  PMID: 6304735

Abstract

Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ambinder J. N., Chiorazzi N., Gibofsky A., Fotino M., Kunkel H. G. Special characteristics of cellular immune function in normal individuals of the HLA-DR3 type. Clin Immunol Immunopathol. 1982 May;23(2):269–274. doi: 10.1016/0090-1229(82)90113-1. [DOI] [PubMed] [Google Scholar]
  2. Cami B., Brégégère F., Abastado J. P., Kourilsky P. Multiple sequences related to classical histocompatibility antigens in the mouse genome. Nature. 1981 Jun 25;291(5817):673–675. doi: 10.1038/291673a0. [DOI] [PubMed] [Google Scholar]
  3. Corte G., Damiani G., Calabi F., Fabbi M., Bargellesi A. Analysis of HLA-DR polymorphism by two-dimensional peptide mapping. Proc Natl Acad Sci U S A. 1981 Jan;78(1):534–538. doi: 10.1073/pnas.78.1.534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  5. Larhammar D., Schenning L., Gustafsson K., Wiman K., Claesson L., Rask L., Peterson P. A. Complete amino acid sequence of an HLA-DR antigen-like beta chain as predicted from the nucleotide sequence: similarities with immunoglobulins and HLA-A, -B, and -C antigens. Proc Natl Acad Sci U S A. 1982 Jun;79(12):3687–3691. doi: 10.1073/pnas.79.12.3687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Larhammar D., Wiman K., Schenning L., Claesson L., Gustafsson K., Peterson P. A., Rask L. Evolutionary relationship between HLA-DR antigen beta-chains, HLA-A, B, C antigen subunits and immunoglobulin chains. Scand J Immunol. 1981 Dec;14(6):617–622. doi: 10.1111/j.1365-3083.1981.tb00603.x. [DOI] [PubMed] [Google Scholar]
  7. Lee J. S., Trowsdale J., Bodmer W. F. cDNA clones coding for the heavy chain of human HLA-DR antigen. Proc Natl Acad Sci U S A. 1982 Jan;79(2):545–549. doi: 10.1073/pnas.79.2.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Legrand L., Rivat-Perran L., Huttin C., Dausset J. HLA-and Gm-linked genes affecting the degradation rate of antigens (sheep red blood cells) endocytized by macrophages. Hum Immunol. 1982 Feb;4(1):1–13. doi: 10.1016/0198-8859(82)90045-3. [DOI] [PubMed] [Google Scholar]
  9. Morling N., Jakobsen B. K., Platz P., Ryder L. P., Svejgaard A., Thomsen M. Typing for human alloantigens with the primed lymphocyte typing technique. Adv Immunol. 1982;32:65–156. doi: 10.1016/s0065-2776(08)60721-x. [DOI] [PubMed] [Google Scholar]
  10. Owerbach D., Bell G. I., Rutter W. J., Shows T. B. The insulin gene is located on chromosome 11 in humans. Nature. 1980 Jul 3;286(5768):82–84. doi: 10.1038/286082a0. [DOI] [PubMed] [Google Scholar]
  11. Owerbach D., Nerup J. Restriction fragment length polymorphism of the insulin gene in diabetes mellitus. Diabetes. 1982 Mar;31(3):275–277. doi: 10.2337/diab.31.3.275. [DOI] [PubMed] [Google Scholar]
  12. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  13. Silver J., Ferrone S. Structural polymorphism of human DR antigens. Nature. 1979 May 31;279(5712):436–437. doi: 10.1038/279436a0. [DOI] [PubMed] [Google Scholar]
  14. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  15. Svejgaard A., Platz P., Ryder L. P., Nielsen L. S., Thomsen M. HL-A and disease associations--a survey. Transplant Rev. 1975;22:3–43. doi: 10.1111/j.1600-065x.1975.tb01550.x. [DOI] [PubMed] [Google Scholar]
  16. Thomsen M., Ryder L. P., Morling N., Platz P., Jakobsen B. K., Svejgaard A. Frequency of nine HLA-D antigens in the Danish population. Tissue Antigens. 1982 Jan;19(1):25–39. doi: 10.1111/j.1399-0039.1982.tb01413.x. [DOI] [PubMed] [Google Scholar]
  17. Thorsby E., Berle E., Nousiainen H. HLA-D region molecules restrict proliferative T cell responses to antigen. Immunol Rev. 1982;66:39–56. doi: 10.1111/j.1600-065x.1982.tb00433.x. [DOI] [PubMed] [Google Scholar]
  18. Wake C. T., Long E. O., Mach B. Allelic polymorphism and complexity of the genes for HLA-DR beta-chains--direct analysis by DNA-DNA hybridization. Nature. 1982 Nov 25;300(5890):372–374. doi: 10.1038/300372a0. [DOI] [PubMed] [Google Scholar]
  19. Wake C. T., Long E. O., Strubin M., Gross N., Accolla R., Carrel S., Mach B. Isolation of cDNA clones encoding HLA-DR alpha chains. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6979–6983. doi: 10.1073/pnas.79.22.6979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wiman K., Larhammar D., Claesson L., Gustafsson K., Schenning L., Bill P., Böhme J., Denaro M., Dobberstein B., Hammerling U. Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1703–1707. doi: 10.1073/pnas.79.6.1703. [DOI] [PMC free article] [PubMed] [Google Scholar]

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