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. 1992 Jul 1;89(13):5966–5970. doi: 10.1073/pnas.89.13.5966

A promoter element that exerts positive and negative control of the interleukin 2-responsive J-chain gene.

R D Lansford 1, H J McFadden 1, S T Siu 1, J S Cox 1, G M Cann 1, M E Koshland 1
PMCID: PMC402119  PMID: 1631082

Abstract

In a primary immune response a signal from interleukin 2 (IL-2) induces B lymphocytes to express the gene for the IgM joining component, the J chain. The signaling mechanism was pursued in this study by examining the J-chain gene 5' flanking region for regulatory sequences and interacting nuclear factors. The analyses identified a major control region located between -75 and -45 that encodes two adjacent elements: a T-rich sequence (JA) containing a single positive regulatory motif and an A+G-rich sequence (JB) containing overlapping positive and negative regulatory motifs. Dissection of the two elements indicated that the bifunctional JB sequence is the likely target of the IL-2 signal. The evidence was based on findings that (i) JB activity correlated with J-chain gene transcription--i.e., JB acts as a repressor in J-chain-silent B cells and as an activator in J-chain-expressing cells, and (ii) JB activator function is mediated by a B-cell-specific nuclear protein, NF-JB, that exhibits an IL-2-responsive binding pattern.

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