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. 1974 Mar;9(3):511–518. doi: 10.1128/iai.9.3.511-518.1974

Isolation of an Acidic Surface Antigen from a Conventional Strain of Staphylococcus aureus

W W Karakawa a, J A Kane a, M R Smith a,1
PMCID: PMC414836  PMID: 4206025

Abstract

Staphylococcus aureus strain 7007, a prototype isolated from a hospital burn unit, was shown to exhibit a significant degree of resistance to ingestion by mouse polymorphonuclear leukocytes. An acidic surface antigen was isolated from strain 7007 by a combination of 10% trichloroacetic acid extraction, ion-exchange chromatography, and gel filtration. Chemical analysis indicated that the surface antigen consists of an unknown aminouronic acid and an amino sugar. Immunochemical analysis suggested that the 7007 antigen is a common feature of all the strains collected from the burn unit. No cross-reactivity was observed between the carbohydrate preparations of various hospital staphylococcal strains and the 7007 heteropolymer. These results suggested the possibility that the surface antigen of strain 7007 represents a strain- or type-specific antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COHN Z. A., MORSE S. I. Interactions between rabbit polymorphonuclear leucocytes and staphylococci. J Exp Med. 1959 Sep 1;110:419–443. doi: 10.1084/jem.110.3.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CURTIS S. N., KRAUSE R. M. ANTIGENIC RELATIONSHIPS BETWEEN GROUPS B AND G STREPTOCOCCI. J Exp Med. 1964 Oct 1;120:629–637. doi: 10.1084/jem.120.4.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ekstedt R. D., Bernhard J. M. Preparation and characterization of a slime layer material produced by Staphylococcus aureus. Proc Soc Exp Biol Med. 1973 Jan;142(1):86–91. doi: 10.3181/00379727-142-36964. [DOI] [PubMed] [Google Scholar]
  4. Ekstedt R. D. Mechanisms of resistance to staphylococcal infection: natural and acquired. Ann N Y Acad Sci. 1965 Jul 23;128(1):301–334. doi: 10.1111/j.1749-6632.1965.tb11646.x. [DOI] [PubMed] [Google Scholar]
  5. HIRSCH J. G., STRAUSS B. STUDIES ON HEAT-LABILE OPSONIN IN RABBIT SERUM. J Immunol. 1964 Jan;92:145–154. [PubMed] [Google Scholar]
  6. Karakawa W. W., Kane J. A. Characterization of the surface antigens of Staphylococcus aureus, strain K-93M. J Immunol. 1972 May;108(5):1199–1208. [PubMed] [Google Scholar]
  7. Karakawa W. W., Krause R. M. Studies on the immunochemistry of streptococcal mucopeptide. J Exp Med. 1966 Aug 1;124(2):155–171. doi: 10.1084/jem.124.2.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Koenig M. G., Melly M. A. The importance of surface antigens in staphylococcal virulence. Ann N Y Acad Sci. 1965 Jul 23;128(1):231–250. doi: 10.1111/j.1749-6632.1965.tb11641.x. [DOI] [PubMed] [Google Scholar]
  9. MCCARTY M., MORSE S. I. CELL WALL ANTIGENS OF GRAM-POSITIVE BACTERIA. Adv Immunol. 1964;27:249–286. doi: 10.1016/s0065-2776(08)60709-9. [DOI] [PubMed] [Google Scholar]
  10. MORSE S. I. DISTRIBUTION OF A SURFACE ANTIGEN OF STAPHYLOCOCCUS AUREUS. Nature. 1963 Dec 14;200:1126–1127. doi: 10.1038/2001126a0. [DOI] [PubMed] [Google Scholar]
  11. MORSE S. I. Isolation and properties of a surface antigen of Staphylococcus aureus. J Exp Med. 1962 Feb 1;115:295–311. doi: 10.1084/jem.115.2.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maverakis N. H., Wiley B. B. Evidence for a multiplicity of capsular types among Staphylococcus aureus strains. J Bacteriol. 1969 Aug;99(2):472–479. doi: 10.1128/jb.99.2.472-479.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McCARTY M., LANCEFIELD R. C. Variation in the group-specific carbohydrate of group A streptococci. I. Immunochemical studies on the carbohydrates of variant strains. J Exp Med. 1955 Jul 1;102(1):11–28. doi: 10.1084/jem.102.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McDonald H. C., Karakawa W. W. Immunochemical analysis of a uronic acid polymer of Staphylococcus epidermidis, strain 53. J Immunol. 1970 Aug;105(2):389–395. [PubMed] [Google Scholar]
  15. Oeding P. Antigenic properties of staphylococci. Ann N Y Acad Sci. 1965 Jul 23;128(1):183–190. doi: 10.1111/j.1749-6632.1965.tb11638.x. [DOI] [PubMed] [Google Scholar]
  16. PERKINS H. R. A polymer containing glucose and aminohexuronic acid isolated from the cell walls of micrococcus lysodeikticus. Biochem J. 1963 Mar;86:475–483. doi: 10.1042/bj0860475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Roberts R. B. The interaction in vitro between group B meningococci and rabbit polymorphonuclear leukocytes. Demonstration of type specific opsonins and bactericidins. J Exp Med. 1967 Nov 1;126(5):795–818. doi: 10.1084/jem.126.5.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rogers D. E., Melly M. A. Speculations on the immunology of staphylococcal infections. Ann N Y Acad Sci. 1965 Jul 23;128(1):274–284. doi: 10.1111/j.1749-6632.1965.tb11644.x. [DOI] [PubMed] [Google Scholar]
  19. Wu T. C., Park J. T. Chemical characterization of a new surface antigenic polysaccharide from a mutant of Staphylococcus aureus. J Bacteriol. 1971 Nov;108(2):874–884. doi: 10.1128/jb.108.2.874-884.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yoshida K. Demonstration of Serologically Different Capsular Types Among Strains of Staphylococcus aureus by the Serum-Soft Agar Technique. Infect Immun. 1971 Apr;3(4):535–539. doi: 10.1128/iai.3.4.535-539.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yoshida K., Ekstedt R. D. Relation of mucoid growth of Staphylococcus aureus to clumping factor reaction, morphology in serum-soft agar, and virulence. J Bacteriol. 1968 Oct;96(4):902–908. doi: 10.1128/jb.96.4.902-908.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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