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. 1973 May;7(5):718–724. doi: 10.1128/iai.7.5.718-724.1973

Ribonucleic Acid and Protein Synthesis in Guinea Pig Liver During Q Fever

H A Thompson a,1, D Paretsky a
PMCID: PMC422750  PMID: 4764402

Abstract

Protein and ribonucleic acid (RNA) syntheses increase in the liver during Q fever, coincident with progressive increments in cortisol levels. Cell-free protein-synthesizing systems indicate that the pH 5 enzyme fraction was chiefly responsible for enhanced activity. Although the polysome profiles from normal and infected livers were similar, the number of liver ribosomes doubled during infection. There was a concomitant increase in orotate incorporation in the 28s, 18s, and 4s RNA species of infected liver.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agarwal M. K., Hanoune J., Yu F. L., Weinstein I. B., Feigelson P. Studies on the effect of cortisone on rat liver transfer ribonucleic acid. Biochemistry. 1969 Dec;8(12):4806–4812. doi: 10.1021/bi00840a023. [DOI] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blobel G., Potter V. R. Relation of ribonuclease and ribonuclease inhibitor to the isolation of polysomes from rat liver. Proc Natl Acad Sci U S A. 1966 May;55(5):1283–1288. doi: 10.1073/pnas.55.5.1283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blobel G., Potter V. R. Studies on free and membrane-bound ribosomes in rat liver. I. Distribution as related to total cellular RNA. J Mol Biol. 1967 Jun 14;26(2):279–292. doi: 10.1016/0022-2836(67)90297-5. [DOI] [PubMed] [Google Scholar]
  5. CRESTFIELD A. M., SMITH K. C., ALLEN F. W. The preparation and characterization of ribonucleic acids from yeast. J Biol Chem. 1955 Sep;216(1):185–193. [PubMed] [Google Scholar]
  6. Clark B. R., Rubin R. T. New fluorometric method for the determination of cortisol in serum. Anal Biochem. 1969 Apr 11;29(1):31–39. doi: 10.1016/0003-2697(69)90004-9. [DOI] [PubMed] [Google Scholar]
  7. Dahmus M. E., Bonner J. Increased template activity of liver chromatin, a result of hydrocortisone administration. Proc Natl Acad Sci U S A. 1965 Nov;54(5):1370–1375. doi: 10.1073/pnas.54.5.1370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Drews J., Brawerman G. Alterations in the nature of ribonucleic acid synthesized in rat liver during regeneration and after cortisol administration. J Biol Chem. 1967 Mar 10;242(5):801–808. [PubMed] [Google Scholar]
  9. FEIGELSON M., GROSS P. R., FEIGELSON P. Early effects of cortisone on nucleic acid and protein metabolism of rat liver. Biochim Biophys Acta. 1962 Apr 2;55:495–504. doi: 10.1016/0006-3002(62)90982-4. [DOI] [PubMed] [Google Scholar]
  10. Finkel R. M., Henshaw E. C., Hiatt H. H. Early changes in liver cytoplasmic RNA of hydrocortisone-treated rats. Mol Pharmacol. 1966 May;2(3):221–226. [PubMed] [Google Scholar]
  11. Greenman D. L., Wicks W. D., Kenney F. T. Stimulation of ribonucleic acid synthesis by steroid hormones. II. High molecular weight components. J Biol Chem. 1965 Nov;240(11):4420–4426. [PubMed] [Google Scholar]
  12. Handley J., Paretsky D., Stueckemann J. Electron microscopic observations of Coxiella burnetii in the guinea pig. J Bacteriol. 1967 Jul;94(1):263–267. doi: 10.1128/jb.94.1.263-267.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hirsch C. A. Quantitative determination of the ribosomal ribonucleic acid content of liver and Novikoff hepatoma from fed and from fasted rats. J Biol Chem. 1967 Jun 25;242(12):2822–2827. [PubMed] [Google Scholar]
  14. KELLER E. B., ZAMECNIK P. C. The effect of guanosine diphosphate and triphosphate on the incorporation of labeled amino acids into proteins. J Biol Chem. 1956 Jul;221(1):45–59. [PubMed] [Google Scholar]
  15. KIDSON C., KIRBY K. S. SELECTIVE ALTERATIONS OF MAMMALIAN MESSENGER-RNA SYNTHESIS: EVIDENCE FOR DIFFERENTIAL ACTION OF HORMONES ON GENE TRANSCRIPTION. Nature. 1964 Aug 8;203:599–603. doi: 10.1038/203599a0. [DOI] [PubMed] [Google Scholar]
  16. KIRBY K. S. ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID. Biochem J. 1965 Jul;96:266–269. doi: 10.1042/bj0960266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kehoe J. M., Lust G. Studies of the host response to infectious disease: alterations of RNA metabolism in mouse tissues. J Infect Dis. 1969 Oct;120(4):411–418. doi: 10.1093/infdis/120.4.411. [DOI] [PubMed] [Google Scholar]
  18. Kenney F. T., Wicks W. D., Greenman D. L. Hydrocortisone stimulation of RNA synthesis in induction of hepatic enzymes. J Cell Physiol. 1965 Oct;66(2 Suppl):125–136. doi: 10.1002/jcp.1030660412. [DOI] [PubMed] [Google Scholar]
  19. LANG N., SEKERKIS C. E. STIMULATION OF RNA-POLYMERASE ACTIVITY IN RAT LIVER BY CORTISOL. Life Sci. 1964 May;3:391–393. doi: 10.1016/0024-3205(64)90198-5. [DOI] [PubMed] [Google Scholar]
  20. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  21. Lust G. Effect of infection on protein and nucleic acid synthesis in mammalian organs and tissues. Fed Proc. 1966 Nov-Dec;25(6):1688–1694. [PubMed] [Google Scholar]
  22. Mallavia L. P., Paretsky D. Physiology of rickettsiae. VII. Amino acid incorporation by Coxiella burnetii and by infected hosts. J Bacteriol. 1967 May;93(5):1479–1483. doi: 10.1128/jb.93.5.1479-1483.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Munro H. N., Fleck A. Recent developments in the measurement of nucleic acids in biological materials. A supplementary review. Analyst. 1966 Feb;91(79):78–88. doi: 10.1039/an9669100078. [DOI] [PubMed] [Google Scholar]
  24. PARETSKY D., DOWNS C. M., SALMON C. W. SOME BIOCHEMICAL CHANGES IN THE GUINEA PIG DURING INFECTION WITH COXIELLA BURNETII. J Bacteriol. 1964 Jul;88:137–142. doi: 10.1128/jb.88.1.137-142.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Paretsky D., Stueckemann J. Chemical and biochemical changes in subcellular fractions of guinea pig liver during infection with Coxiella burneti. J Bacteriol. 1970 May;102(2):334–340. doi: 10.1128/jb.102.2.334-340.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shtasel T. F., Berry L. J. Effect of endotoxin and cortisone on synthesis of ribonucleic acid and protein in livers of mice. J Bacteriol. 1969 Mar;97(3):1018–1025. doi: 10.1128/jb.97.3.1018-1025.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Stueckemann J., Paretsky D. Changes in hepatic glycogen, protein, and ribonucleic acid synthesis, and some effects of cortisol, during Q fever. J Bacteriol. 1971 Jun;106(3):920–924. doi: 10.1128/jb.106.3.920-924.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Thompson H. A., Baca O. G., Paretsky D. Presence of ribosomal ribonucleic acid in the rickettsia Coxiella burneti. Biochem J. 1971 Nov;125(1):365–366. doi: 10.1042/bj1250365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. WETTSTEIN F. O., STAEHELIN T., NOLL H. Ribosomal aggregate engaged in protein synthesis: characterization of the ergosome. Nature. 1963 Feb 2;197:430–435. doi: 10.1038/197430a0. [DOI] [PubMed] [Google Scholar]
  30. Yu F. L., Feigelson P. Cortisone stimulation of nucleolar RNA polymerase activity. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2177–2180. doi: 10.1073/pnas.68.9.2177. [DOI] [PMC free article] [PubMed] [Google Scholar]

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