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. 1981 Mar;67(3):530–534. doi: 10.1104/pp.67.3.530

Similarity of Ribulose-1,5-bisphosphate Carboxylases of Isogenic Diploid and Tetraploid Ryegrass (Lolium perenne L.) Cultivars 1

Philip H McNeil 1,2,2, Christine H Foyer 1,2, David A Walker 1,2,3, Ivan F Bird 1,2, Martin J Cornelius 1,2, Alfred J Keyes 1,2
PMCID: PMC425719  PMID: 16661708

Abstract

Partially purified ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39) was isolated from diploid and tetraploid cultivars of ryegrass (Lolium perenne L.) using two separate methods. The apparent Km (CO2) values for the enzymes prepared by either method did not differ significantly between diploid and tetraploid when assayed by two separate techniques. The unpurified enzymes from freshly lysed (and fully functional) protoplasts of both diploid and tetraploid cultivars gave virtually identical apparent Km (CO2) values. There was no indication of large differences in affinity for CO2 of illuminated intact protoplasts from the two cultivars.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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