Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1982 Apr;69(4):983–987. doi: 10.1104/pp.69.4.983

Glutamine Synthetase in Spinach Leaves 1

IMMUNOLOGICAL STUDIES AND IMMUNOCYTOCHEMICAL LOCALIZATION

Bertrand Hirel 1,2, Catherine Perrot-Rechenmann 1, Akira Suzuki 1, Jean Vidal 1, Pierre Gadal 1
PMCID: PMC426340  PMID: 16662331

Abstract

By polyacrylamide gel electrophoresis, DEAE Sephacel, and hydroxyapatite chromatography, one form of glutamine synthetase has been identified in spinach (Spinacia oleracea L. cv. Monstrueux de Viroflay) leaves. It is localized only inside the chloroplast. The enzyme was purified to homogeneity and specific antibodies against the protein were raised by immunization of rabbits. The intracellular localization of glutamine synthetase in spinach leaves was studied by indirect immunofluorescence microscopy on thin-sectioned spinach leaves. It has been demonstrated that the enzyme is specifically associated with the chloroplasts of parenchymatous cells.

Full text

PDF
983

Images in this article

985Image
on p.985
985Image
on p.985
986Image
on p.986

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. W., Done J. Polarographic study of ammonia assimilation by isolated chloroplasts. Plant Physiol. 1977 Oct;60(4):504–508. doi: 10.1104/pp.60.4.504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cockburn W., Walker D. A., Baldry C. W. The isolation of spinach chloroplasts in pyrophosphate media. Plant Physiol. 1968 Sep;43(9):1415–1418. doi: 10.1104/pp.43.9.1415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hirel B., Gadal P. Glutamine Synthetase in Rice: A COMPARATIVE STUDY OF THE ENZYMES FROM ROOTS AND LEAVES. Plant Physiol. 1980 Oct;66(4):619–623. doi: 10.1104/pp.66.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MAYOR H. D., HAMPTON J. C., ROSARIO B. A simple method for removing the resin from epoxy-embedded tissue. J Biophys Biochem Cytol. 1961 Apr;9:909–910. doi: 10.1083/jcb.9.4.909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mann A. F., Fentem P. A., Stewart G. R. Identification of two forms of glutamine synthetase in barley (Hordeum vulgare). Biochem Biophys Res Commun. 1979 May 28;88(2):515–521. doi: 10.1016/0006-291x(79)92078-3. [DOI] [PubMed] [Google Scholar]
  7. Mann A. F., Fentem P. A., Stewart G. R. Tissue localization of barley (Hordeum vulgare) glutamine synthetase isoenzymes. FEBS Lett. 1980 Feb 11;110(2):265–267. doi: 10.1016/0014-5793(80)80088-3. [DOI] [PubMed] [Google Scholar]
  8. Mitchell C. A., Stocking C. R. Kinetics and Energetics of Light-driven Chloroplast Glutamine Synthesis. Plant Physiol. 1975 Jan;55(1):59–63. doi: 10.1104/pp.55.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. O'Neal D., Joy K. W. Localisation of glutamine synthetase in chloroplasts. Nat New Biol. 1973 Nov 14;246(150):61–62. doi: 10.1038/newbio246061a0. [DOI] [PubMed] [Google Scholar]
  10. Okamoto K., Akazawa T. Enzymic Mechanism of Starch Breakdown in Germinating Rice Seeds: 8. Immunohistochemical Localization of beta-Amylase. Plant Physiol. 1979 Aug;64(2):337–340. doi: 10.1104/pp.64.2.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sayre R. T., Kennedy R. A. Photosynthetic Enzyme Activities and Localization in Mollugo verticillata Populations Differing in the Levels of C(3) and C(4) Cycle Operation. Plant Physiol. 1979 Aug;64(2):293–299. doi: 10.1104/pp.64.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Scopes R. K. Measurement of protein by spectrophotometry at 205 nm. Anal Biochem. 1974 May;59(1):277–282. doi: 10.1016/0003-2697(74)90034-7. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES