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. 1982 Apr;69(4):983–987. doi: 10.1104/pp.69.4.983

Glutamine Synthetase in Spinach Leaves 1

IMMUNOLOGICAL STUDIES AND IMMUNOCYTOCHEMICAL LOCALIZATION

Bertrand Hirel 1,2, Catherine Perrot-Rechenmann 1, Akira Suzuki 1, Jean Vidal 1, Pierre Gadal 1
PMCID: PMC426340  PMID: 16662331

Abstract

By polyacrylamide gel electrophoresis, DEAE Sephacel, and hydroxyapatite chromatography, one form of glutamine synthetase has been identified in spinach (Spinacia oleracea L. cv. Monstrueux de Viroflay) leaves. It is localized only inside the chloroplast. The enzyme was purified to homogeneity and specific antibodies against the protein were raised by immunization of rabbits. The intracellular localization of glutamine synthetase in spinach leaves was studied by indirect immunofluorescence microscopy on thin-sectioned spinach leaves. It has been demonstrated that the enzyme is specifically associated with the chloroplasts of parenchymatous cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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