Abstract
Ribosomes containing 5S RNA in which the 3′-terminus has been modified chemically, either by periodate oxidation or by conversion of the terminal ribose to a morpholine derivative, are fully as active in peptidebond formation and polypeptide synthesis as those ribosomes with unmodified 5S RNA. These results exclude models for protein synthesis in which peptidyl-5S RNA is formed as an intermediate.
Keywords: B. stearothermophilus, peptidyl-tRNA, periodate, morpholine derivative, protein synthesis
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Erdmann V. A., Fahnestock S., Higo K., Nomura M. Role of 5S RNA in the functions of 50S ribosomal subunits. Proc Natl Acad Sci U S A. 1971 Dec;68(12):2932–2936. doi: 10.1073/pnas.68.12.2932. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KHYM J. X. The reaction of methylamine with periodate-oxidized adenosine 5'-phosphate. Biochemistry. 1963 Mar-Apr;2:344–350. doi: 10.1021/bi00902a029. [DOI] [PubMed] [Google Scholar]
- Miskin R., Zamir A., Elson D. Inactivation and reactivation of ribosomal subunits: the peptidyl transferase activity of the 50 s subunit of Escherihia coli. J Mol Biol. 1970 Dec 14;54(2):355–378. doi: 10.1016/0022-2836(70)90435-3. [DOI] [PubMed] [Google Scholar]
- NEU H. C., HEPPEL L. A. NUCLEOTIDE SEQUENCE ANALYSIS OF POLYRIBONUCLEOTIDES BY MEANS OF PERIODATE OXIDATION FOLLOWED BY CLEAVAGE WITH AN AMINE. J Biol Chem. 1964 Sep;239:2927–2934. [PubMed] [Google Scholar]
- Raacke I. D. A model for protein synthesis involving the intermediate formation of peptidyl-5S RNA. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2357–2360. doi: 10.1073/pnas.68.10.2357. [DOI] [PMC free article] [PubMed] [Google Scholar]