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. 1972 Mar;69(3):752–755. doi: 10.1073/pnas.69.3.752

Elongation Factors EF Tu and EF G Interact at Related Sites on Ribosomes

David Lee Miller 1
PMCID: PMC426550  PMID: 4551986

Abstract

The binding of elongation factor EF G to ribosomes inhibits the subsequent reaction of the ribosomes with the ternary complex aminoacyl-tRNA·EF Tu·GTP. Both the hydrolysis of GTP and the binding of aminoacyl-tRNA to ribosomes are nearly abolished by the previous binding of factor EF G to ribosomes in the presence of either fusidic acid plus either GTP or a nonhydrolyzable analog of GTP. The results suggest that each elongation factor binds to the same region on the ribosome. The GTPase activities of both factors EF G and EF Tu may be activated by interaction at the same ribosomal site, as has been previously suggested by others.

Keywords: aminoacyl-tRNA binding, GTP hydrolysis, fusidic acid, chloramphenicol, GMPPCP

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bodley J. W., Zieve F. J., Lin L. Studies on translocation. IV. The hydrolysis of a single round of guanosine triphosphate in the presence of fusidic acid. J Biol Chem. 1970 Nov 10;245(21):5662–5667. [PubMed] [Google Scholar]
  2. Brot N., Spears C., Weissbach H. The formation of a complex containing ribosomes, transfer factor G and A guanosine nucleotide. Biochem Biophys Res Commun. 1969 Mar 31;34(6):843–848. doi: 10.1016/0006-291x(69)90257-5. [DOI] [PubMed] [Google Scholar]
  3. Brot N., Spears C., Weissbach H. The interaction of transfer factor G, ribosomes, and guanosine nucleotides in the presence of fusidic acid. Arch Biochem Biophys. 1971 Mar;143(1):286–296. doi: 10.1016/0003-9861(71)90211-6. [DOI] [PubMed] [Google Scholar]
  4. Brot N., Yamasaki E., Redfield B., Weissbach H. The binding of aminoacyl-tRNA and poly U to a soluble factor (S) extracted from ribosomes. Biochem Biophys Res Commun. 1970 Aug 11;40(3):698–707. doi: 10.1016/0006-291x(70)90960-5. [DOI] [PubMed] [Google Scholar]
  5. Cabrer B., Vázquez D., Modolell J. Inhibition by elongation factor EF G of aminoacyl-tRNA binding to ribosomes. Proc Natl Acad Sci U S A. 1972 Mar;69(3):733–736. doi: 10.1073/pnas.69.3.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gordon J. Hydrolysis of guanosine 5'-triphosphate associated wh binding of aminoacyl transfer ribonucleic acid to ribosomes. J Biol Chem. 1969 Oct 25;244(20):5680–5686. [PubMed] [Google Scholar]
  7. Gupta S. L., Waterson J., Sopori M. L., Weissman S. M., Lengyel P. Movement of the ribosome along the messenger ribonucleic acid during protein synthesis. Biochemistry. 1971 Nov 23;10(24):4410–4421. doi: 10.1021/bi00800a010. [DOI] [PubMed] [Google Scholar]
  8. Highland J. H., Lin L., Bodley J. W. Protection of ribosomes from thiostrepton inactivation by the binding of G factor and guanosine diphosphate. Biochemistry. 1971 Nov 23;10(24):4404–4409. doi: 10.1021/bi00800a009. [DOI] [PubMed] [Google Scholar]
  9. Ishitsuka H., Kuriki Y., Kaji A. Release of transfer ribonucleic acid from ribosomes. A G factor and guanosine triphosphate-dependent reaction. J Biol Chem. 1970 Jul 10;245(13):3346–3351. [PubMed] [Google Scholar]
  10. Kinoshita T., Liou Y., Tanaka N. Inhibition by thiopeptin of ribosomal functions associated with T and G factors. Biochem Biophys Res Commun. 1971 Aug 20;44(4):859–863. doi: 10.1016/0006-291x(71)90790-x. [DOI] [PubMed] [Google Scholar]
  11. Lucas-Lenard J. Protein biosynthesis. Annu Rev Biochem. 1971;40:409–448. doi: 10.1146/annurev.bi.40.070171.002205. [DOI] [PubMed] [Google Scholar]
  12. Miller D. L., Weissbach H. Studies on the purification and properties of factor Tu from E. coli. Arch Biochem Biophys. 1970 Nov;141(1):26–37. doi: 10.1016/0003-9861(70)90102-5. [DOI] [PubMed] [Google Scholar]
  13. Modolell J., Cabrer B., Parmeggiani A., Vazquez D. Inhibition by siomycin and thiostrepton of both aminoacyl-tRNA and factor G binding to ribosomes. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1796–1800. doi: 10.1073/pnas.68.8.1796. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Parmeggiani A., Gottschalk E. M. Properties of the crystalline amino acid polymerization factors from Escherichia coli: binding of G to ribosomes. Biochem Biophys Res Commun. 1969 Jun 27;35(6):861–867. doi: 10.1016/0006-291x(69)90703-7. [DOI] [PubMed] [Google Scholar]
  15. Richman N., Bodley J. W. Ribosomes cannot interact simultaneously with elongation factors EF Tu and EF G. Proc Natl Acad Sci U S A. 1972 Mar;69(3):686–689. doi: 10.1073/pnas.69.3.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shafritz D., Goodwin F., Weissbach H. Inhibition by aminochromes of in vitro polypeptide synthesis in Escherichia coli. Arch Biochem Biophys. 1969 Nov;134(2):478–485. doi: 10.1016/0003-9861(69)90308-7. [DOI] [PubMed] [Google Scholar]
  17. Shorey R. L., Ravel J. M., Garner C. W., Shive W. Formation and properties of the aminoacyl transfer ribonucleic acid-guanosine triphosphate-protein complex. J Biol Chem. 1969 Sep 10;244(17):4555–4564. [PubMed] [Google Scholar]
  18. Skoultchi A., Ono Y., Waterson J., Lengyel P. Peptide chain elongation; indications for the binding of an amino acid polymerization factor, guanosine 5'-triphosphate--aminoacyl transfer ribonucleic acid complex to the messenger-ribosome complex. Biochemistry. 1970 Feb 3;9(3):508–514. doi: 10.1021/bi00805a009. [DOI] [PubMed] [Google Scholar]
  19. Thach S. S., Thach R. E. Translocation of messenger RNA and "accommodation" of fMet-tRNA. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1791–1795. doi: 10.1073/pnas.68.8.1791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weissbach H., Miller D. L., Hachmann J. Studies on the role of factor Ts in polypeptide synthesis. Arch Biochem Biophys. 1970 Mar;137(1):262–269. doi: 10.1016/0003-9861(70)90433-9. [DOI] [PubMed] [Google Scholar]
  21. Weissbach H., Redfield B., Brot N. Aminoacyl-tRNA-Tu-GTP interaction with ribosomes. Arch Biochem Biophys. 1971 Aug;145(2):676–684. doi: 10.1016/s0003-9861(71)80028-0. [DOI] [PubMed] [Google Scholar]

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