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. 1972 Jun;69(6):1620–1624. doi: 10.1073/pnas.69.6.1620

Fractionation of Antigen Reactive Cells on a Cellular Immunoadsorbent: Factors Determining Recognition of Antigens by T-Lymphocytes

H Wekerle 1,*, P Lonai 1,†, M Feldman 1
PMCID: PMC426761  PMID: 4504379

Abstract

Monolayers of mouse fibroblasts were used as cellular immunoadsorbents to separate rat lymphocytes that recognize specific mouse histocompatibility antigens. Normal lymphocytes were incubated on fibroblasts of strain C3H/eb, and nonadherent cells were separated from adherent cells, then transferred for sensitization onto fresh monolayers of C3H. When tested on 51Cr-labeled target monolayers the nonadherent cells manifested significantly lower cytotoxicity than the adherent cells. However, the nonadherent cells could be sensitized against mouse fibroblasts of an unrelated H-2 phenotype (strain Balb/c). The immune specificity of the adherence was further demonstrated by a stepwise adsorption, which resulted in complete loss of the capacity to acquire specific lytic activity towards C3H antigens. Lymphocytes recognizing strain-specific antigens of the mouse were separated at 37° (not at 0° or 4°); separation was inhibited after treatment with dinitrophenol, sodium azide, or trypsin. Prior treatment with neuraminidase rendered the lymphocytes suceptible to separation at 0°.

Keywords: mouse fibroblasts, cell receptors, cell culture

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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