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. 1973 Oct;70(10):2911–2915. doi: 10.1073/pnas.70.10.2911

Isolation of Escherichia coli RNA Polymerase Binding Sites on T5 and T7 DNA: Further Evidence for Sigma-Dependent Recognition of A-T-Rich DNA Sequences

J Y Le Talaer 1,2, M Kermici 1,2, Ph Jeanteur 1,2
PMCID: PMC427137  PMID: 4355373

Abstract

Previous isolation and analysis of E. coli RNA polymerase (EC 2.7.7.6) binding sites on λ DNA had demonstrated the existence of a sigma-dependent process of recognition of A-T-rich DNA sequences. We have now extended this finding to T5 and T7 DNA and häve provided evidence for the double-strandedness of the isolated binding sites. The possible equation of these sites to the genetically defined promoters is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Babinet C. A new method for the purification of RNA-polymerase. Biochem Biophys Res Commun. 1967 Mar 21;26(6):639–644. doi: 10.1016/s0006-291x(67)80119-0. [DOI] [PubMed] [Google Scholar]
  2. Bautz E. K., Bautz F. A. Initiation of RNA synthesis: the function of sigma in the binding of RNA polymerase to promoter sites. Nature. 1970 Jun 27;226(5252):1219–1222. doi: 10.1038/2261219a0. [DOI] [PubMed] [Google Scholar]
  3. Blattner F. R., Dahlberg J. E., Boettiger J. K., Fiandt M., Szybalski W. Distance from a promoter mutation to an RNA synthesis startpoint on bacteriophage lambda DNA. Nat New Biol. 1972 Jun 21;237(77):232–236. doi: 10.1038/newbio237232a0. [DOI] [PubMed] [Google Scholar]
  4. Blattner F. R., Dahlberg J. E. RNA synthesis startpoints in bacteriophage lambda: are the promoter and operator transcribed? Nat New Biol. 1972 Jun 21;237(77):227–232. doi: 10.1038/newbio237227a0. [DOI] [PubMed] [Google Scholar]
  5. Bram S. The function of the structure of DNA in chromosomes. Biochimie. 1972;54(8):1005–1011. doi: 10.1016/s0300-9084(72)80051-8. [DOI] [PubMed] [Google Scholar]
  6. Burgess R. R. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6160–6167. [PubMed] [Google Scholar]
  7. Dausse J. P., Sentenac A., Fromageot P. Interaction of RNA polymerase from Escherichia coli with DNA. Selection of initiation sites on T 7 DNA. Eur J Biochem. 1972 Mar 15;26(1):43–49. doi: 10.1111/j.1432-1033.1972.tb01737.x. [DOI] [PubMed] [Google Scholar]
  8. Heyden B., Nüsslein C., Schaller H. Single RNA polymerase binding site isolated. Nat New Biol. 1972 Nov 1;240(96):9–12. doi: 10.1038/newbio240009a0. [DOI] [PubMed] [Google Scholar]
  9. Hinkle D. C., Chamberlin M. J. Studies of the binding of Escherichia coli RNA polymerase to DNA. I. The role of sigma subunit in site selection. J Mol Biol. 1972 Sep 28;70(2):157–185. doi: 10.1016/0022-2836(72)90531-1. [DOI] [PubMed] [Google Scholar]
  10. Jones O. W., Berg P. Studies on the binding of RNA polymerase to polynucleotides. J Mol Biol. 1966 Dec 28;22(2):199–209. doi: 10.1016/0022-2836(66)90126-4. [DOI] [PubMed] [Google Scholar]
  11. LANNI Y. T. Invasion by bacteriophage T5. III. Stages revealed by changes in susceptibility of early complexes to abortive infection. Virology. 1961 Oct;15:127–135. doi: 10.1016/0042-6822(61)90229-x. [DOI] [PubMed] [Google Scholar]
  12. LINN S., LEHMAN I. R. AN ENDONUCLEASE FROM NEUROSPORA CRASSA SPECIFIC FOR POLYNUCLEOTIDES LACKING AN ORDERED STRUCTURE. I. PURIFICATION AND PROPERTIES OF THE ENZYME. J Biol Chem. 1965 Mar;240:1287–1293. [PubMed] [Google Scholar]
  13. Le Talaer J. Y., Jeanteur P. Discrimination by tRNA of two types of isolated binding sites for E. Coli RNA-polymerase on phage lambda DNA. FEBS Lett. 1972 Dec 15;28(3):305–308. doi: 10.1016/0014-5793(72)80737-3. [DOI] [PubMed] [Google Scholar]
  14. Le Talaer J. Y., Jeanteur P. Preferential binding of E. coli RNA-polymerase to A-T rich sequences of bacteriophage lambda DNA. FEBS Lett. 1971 Jan 30;12(5):253–256. doi: 10.1016/0014-5793(71)80190-4. [DOI] [PubMed] [Google Scholar]
  15. Le Talaer J. Y., Jeanteur P. Purification and base composition analysis of phage lambda early promoters. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3211–3215. doi: 10.1073/pnas.68.12.3211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LePecq J. B., Baldwin R. L. The starting point and direction of lambda DNA replication. Cold Spring Harb Symp Quant Biol. 1968;33:609–620. doi: 10.1101/sqb.1968.033.01.067. [DOI] [PubMed] [Google Scholar]
  17. Okamoto T., Sugiura M., Takanami M. RNA polymerase binding sites of phage fd replicative form DNA. Nat New Biol. 1972 May 24;237(73):108–109. doi: 10.1038/newbio237108a0. [DOI] [PubMed] [Google Scholar]
  18. Saucier J. M., Wang J. C. Angular alteration of the DNA helix by E. coli RNA polymerase. Nat New Biol. 1972 Oct 11;239(93):167–170. doi: 10.1038/newbio239167a0. [DOI] [PubMed] [Google Scholar]
  19. Stead N. W., Jones O. W. Stability of RNA polymerase--DNA complexes. J Mol Biol. 1967 May 28;26(1):131–135. doi: 10.1016/0022-2836(67)90267-7. [DOI] [PubMed] [Google Scholar]

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