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. 1973 Dec;70(12 Pt 1-2):3541–3545. doi: 10.1073/pnas.70.12.3541

Marker Discrimination in Transformation and Mutation of Pneumococcus

J-G Tiraby 1, M S Fox 1
PMCID: PMC427276  PMID: 4148702

Abstract

Earlier investigations of pneumococcal transformation revealed a function (hex+) responsible for severely reducing the transformation yield of certain markers. A mutational alteration (hex-) responsible for the loss of this function has been transferred into a hex+ strain to permit a comparison of the hex+ and hex- phenotypes in an isogenic background.

The loss of the hex+ function results both in a loss of the capacity to eliminate the low efficiency markers in transformation and a substantial increase in the spontaneous mutation rate. These properties of the hex- strain could result from the loss of a capacity to eliminate certain classes of mismatched base pairs that occur as intermediates in both transformation and mutagenesis.

Keywords: bacterial transformation, spontaneous mutation, mismatched base pairs

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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