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. 1975 Jul;8(1):91–94. doi: 10.1128/aac.8.1.91

Rapid Detection of Ampicillin-Resistant Haemophilus influenzae and Their Susceptibility to Sixteen Antibiotics

Robert B Kammer 1, David A Preston 1, Jan R Turner 1, Lois C Hawley 1
PMCID: PMC429266  PMID: 1080656

Abstract

Ampicillin-resistant and -susceptible strains of Haemophilus influenzae were tested for susceptibility to 16 antibiotics. Chloramphenicol and a new cephalosporin, cefamandole, were most active with minimal inhibitory concentrations (MICs) for all bacteria tested between 0.5 to 2.0 μg/ml. All but two organisms were susceptible to tetracycline. Ampicillin-resistant strains of H. influenzae were less susceptible (MIC, 4 to 32 μg/ml) to carbenicillin and ticarcillin than ampicillin-susceptible organisms (MIC, 0.25 to 1.0 μg/ml). A rapid assay for β-lactamase, utilizing a chromogenic cephalosporin substrate, detected enzyme production in all 17 ampicillin-resistant strains of H. influenzae.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Farrar W. E., Jr, O'Dell N. M. Beta-lactamase activity in ampicillin-resistant Haemophilus influenzae. Antimicrob Agents Chemother. 1974 Nov;6(5):625–629. doi: 10.1128/aac.6.5.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Khan W., Ross S., Rodriguez W., Controni G., Saz A. K. Haemophilus influenzae type B resistant to ampicillin. A report of two cases. JAMA. 1974 Jul 15;229(3):298–301. [PubMed] [Google Scholar]
  3. NOVICK R. P. Micro-iodometric assay for penicillinase. Biochem J. 1962 May;83:236–240. doi: 10.1042/bj0830236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Nelson J. D. Editorial: Should ampicillin be abandoned for treatment of Haemophilus influenzae disease? JAMA. 1974 Jul 15;229(3):322–324. [PubMed] [Google Scholar]
  5. O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Stewart S. M. Letter: Ampicillin-resistant Haemophilus influenzae. Lancet. 1974 Jun 8;1(7867):1163–1164. doi: 10.1016/s0140-6736(74)90646-1. [DOI] [PubMed] [Google Scholar]
  7. Thomas W. J., McReynolds J. W., Mock C. R., Bailey D. W. Letter: Ampicillin-resistant Haemophilus influenzae meningitis. Lancet. 1974 Feb 23;1(7852):313–313. doi: 10.1016/s0140-6736(74)92617-8. [DOI] [PubMed] [Google Scholar]
  8. Thornsberry C., Kirven L. A. Ampicillin resistance in Haemophilus influenzae as determined by a rapid test for beta-lactamase production. Antimicrob Agents Chemother. 1974 Nov;6(5):653–654. doi: 10.1128/aac.6.5.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Thornsberry C., Kirven L. A. Antimicrobial susceptibility of Haemophilus influenzae. Antimicrob Agents Chemother. 1974 Nov;6(5):620–624. doi: 10.1128/aac.6.5.620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tomeh M. O., Starr S. E., McGowan J. E., Jr, Terry P. M., Nahmias A. J. Ampicillin-resistant Haemophilus influenzae type B infection. JAMA. 1974 Jul 15;229(3):295–297. [PubMed] [Google Scholar]

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