Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1977 Aug;12(2):298–300. doi: 10.1128/aac.12.2.298

Cytotoxicity and Cell Cycle Specificity of Homoharringtonine

D M Baaske 1, Peter Heinstein 1
PMCID: PMC429904  PMID: 900926

Abstract

The plant alkaloid, homoharringtonine, isolated from Cephalotaxus harringtonia is cytotoxic to HeLa, KB, and L cells growing in monolayer cell cultures. This effect appears to be cell-cycle specific. In synchronized KB cells, protein synthesis was preferentially inhibited in the G1 and G2 phases (70 and 45% inhibition, respectively) as might be expected for a protein-synthesis inhibitor.

Full text

PDF
300

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BASERGA R. THE RELATIONSHIP OF THE CELL CYCLE TO TUMOR GROWTH AND CONTROL OF CELL DIVISION: A REVIEW. Cancer Res. 1965 Jun;25:581–595. [PubMed] [Google Scholar]
  2. BOOTSMA D., BUDKE L., VOS O. STUDIES ON SYNCHRONOUS DIVISION OF TISSUE CULTURE CELLS INITIATED BY EXCESS THYMIDINE. Exp Cell Res. 1964 Jan;33:301–309. doi: 10.1016/s0014-4827(64)81035-1. [DOI] [PubMed] [Google Scholar]
  3. Cohen G. H., Vaughan R. K., Lawrence W. C. Deoxyribonucleic acid synthesis in synchronized mammalian KB cells infected with herpes simplex virus. J Virol. 1971 Jun;7(6):783–791. doi: 10.1128/jvi.7.6.783-791.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DeVita V. T., Jr, Young R. C., Canellos G. P. Combination versus single agent chemotherapy: a review of the basis for selection of drug treatment of cancer. Cancer. 1975 Jan;35(1):98–110. doi: 10.1002/1097-0142(197501)35:1<98::aid-cncr2820350115>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  5. EAGLE H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med. 1955 Jul;89(3):362–364. doi: 10.3181/00379727-89-21811. [DOI] [PubMed] [Google Scholar]
  6. Huang M. T. Harringtonine, an inhibitor of initiation of protein biosynthesis. Mol Pharmacol. 1975 Sep;11(5):511–519. [PubMed] [Google Scholar]
  7. OYAMA V. I., EAGLE H. Measurement of cell growth in tissue culture with a phenol reagent (folin-ciocalteau). Proc Soc Exp Biol Med. 1956 Feb;91(2):305–307. doi: 10.3181/00379727-91-22245. [DOI] [PubMed] [Google Scholar]
  8. PUCK T. T., STEFFEN J. LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION. Biophys J. 1963 Sep;3:379–397. doi: 10.1016/s0006-3495(63)86828-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Powell R. G., Weisleder D., Smith C. R., Jr Antitumor alkaloids for Cephalataxus harringtonia: structure and activity. J Pharm Sci. 1972 Aug;61(8):1227–1230. doi: 10.1002/jps.2600610812. [DOI] [PubMed] [Google Scholar]
  10. Powell R. G., Weisleder D., Smith C. R., Jr, Rohwedder W. K. Structures of harringtonine, isoharringtonine, and homoharringtonine. Tetrahedron Lett. 1970 Mar;(11):815–818. doi: 10.1016/s0040-4039(01)97839-6. [DOI] [PubMed] [Google Scholar]
  11. Puck T. T. Phasing, Mitotic Delay, and Chromosomal Aberrations in Mammalian Cells. Science. 1964 May 1;144(3618):565–566. doi: 10.1126/science.144.3618.565-c. [DOI] [PubMed] [Google Scholar]
  12. XEROS N. Deoxyriboside control and synchronization of mitosis. Nature. 1962 May 19;194:682–683. doi: 10.1038/194682a0. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES