Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Sep;73(9):3156–3159. doi: 10.1073/pnas.73.9.3156

DNA dependent synthesis of protein L12 from escherichia coli ribosomes, in vitro.

F Chu, H F Kung, P Caldwell, H Weissbach, N Brot
PMCID: PMC430964  PMID: 787986

Abstract

The in vitro synthesis of ribosomal protein L12 has been obtained in a coupled system with DNA extracted from the transducing phage lambdarifd18 as template. In addition, a second protein (molecular weight of 16,000) with immunological, chemical, and ribosome-binding characteristics similar to L12 is formed in this in vitro system. The synthesis of both proteins is depressed by one-half when guanosine-5'-diphosphate-3'-diphosphate is added to the reaction mixture.

Full text

PDF
3157

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aboud M., Pastan I. Stimulation of lac transcription by guanosine 5'-diphosphate 2'(or 3')-diphosphate and transfer ribonucleic acid. J Biol Chem. 1973 May 10;248(9):3356–3358. [PubMed] [Google Scholar]
  2. Brot N., Marcel R., Yamasaki E., Weissbach H. Further studies on the role of 50 S ribosomal proteins in protein synthesis. J Biol Chem. 1973 Oct 25;248(20):6952–6956. [PubMed] [Google Scholar]
  3. Brot N., Marcel R., Yamasaki E., Weissbach H. Further studies on the role of 50 S ribosomal proteins in protein synthesis. J Biol Chem. 1973 Oct 25;248(20):6952–6956. [PubMed] [Google Scholar]
  4. Dennis P. P., Nomura M. Stringent control of ribosomal protein gene expression in Escherichia coli. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3819–3823. doi: 10.1073/pnas.71.10.3819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dennis P. P., Nomura M. Stringent control of the transcriptional activities of ribosomal protein genes in E. coli. Nature. 1975 Jun 5;255(5508):460–465. doi: 10.1038/255460a0. [DOI] [PubMed] [Google Scholar]
  6. Deusser E., Weber J., Maschler R., Stöffler G., Wittmann H. G. Structural and functional evidence for a repeated 50S subunit ribosomal protein. Nat New Biol. 1973 Mar 14;242(115):47–49. doi: 10.1038/newbio242047a0. [DOI] [PubMed] [Google Scholar]
  7. Furano A. V., Wittel F. P. Syntheses of elongation factors Tu and G are under stringent control in Escherichia coli. J Biol Chem. 1976 Feb 10;251(3):898–901. [PubMed] [Google Scholar]
  8. Hardy S. J. The stoichiometry of the ribosomal proteins of Escherichia coli. Mol Gen Genet. 1975 Oct 3;140(3):253–274. doi: 10.1007/BF00334270. [DOI] [PubMed] [Google Scholar]
  9. Jaskunas S. R., Lindahl L., Nomura M. Identification of two copies of the gene for the elongation factor EF-Tu in E. coli. Nature. 1975 Oct 9;257(5526):458–462. doi: 10.1038/257458a0. [DOI] [PubMed] [Google Scholar]
  10. Kirschbaum J. B., Konrad E. B. Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase. J Bacteriol. 1973 Nov;116(2):517–526. doi: 10.1128/jb.116.2.517-526.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kung H. F., Brot N., Spears C., Chen B., Weissbach H. Studies on the in vitro transcription and translation of the lac operon. Arch Biochem Biophys. 1974 Jan;160(1):168–174. doi: 10.1016/s0003-9861(74)80023-8. [DOI] [PubMed] [Google Scholar]
  12. Kung H., Spears C., Weissbach H. Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase. J Biol Chem. 1975 Feb 25;250(4):1556–1562. [PubMed] [Google Scholar]
  13. LEBOY P. S., COX E. C., FLAKS J. G. THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1964 Dec;52:1367–1374. doi: 10.1073/pnas.52.6.1367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Lindahl L., Jaskunas S. R., Dennis P. P., Nomura M. Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2743–2747. doi: 10.1073/pnas.72.7.2743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lund E., Dahlberg J. E., Lindahl L., Jaskunas S. R., Dennis P. P., Nomura M. Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. coli. Cell. 1976 Feb;7(2):165–177. doi: 10.1016/0092-8674(76)90016-7. [DOI] [PubMed] [Google Scholar]
  18. Morrissey J. J., Weissbach H., Brot N. The identification and characterization of proteins similar to L7, L12 in ribosome-free extracts of Escherichia coli. Biochem Biophys Res Commun. 1975 Jul 8;65(1):293–302. doi: 10.1016/s0006-291x(75)80092-1. [DOI] [PubMed] [Google Scholar]
  19. Möller W., Groene A., Terhorst C., Amons R. 50-S ribosomal proteins. Purification and partial characterization of two acidic proteins, A 1 and A 2, isolated from 50-S ribosomes of Escherichia coli. Eur J Biochem. 1972 Jan 31;25(1):5–12. doi: 10.1111/j.1432-1033.1972.tb01660.x. [DOI] [PubMed] [Google Scholar]
  20. Reiness G., Yang H. L., Zubay G., Cashel M. Effects of guanosine tetraphosphate on cell-free synthesis of Escherichia coli ribosomal RNA and other gene products. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2881–2885. doi: 10.1073/pnas.72.8.2881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Smolin D. E., Umbarger H. E. Specificity of the stimulation of in vitro ribonucleic acid synthesis by guanosine 5'-diphosphate 3'-diphosphate. Mol Gen Genet. 1975 Dec 9;141(4):277–284. doi: 10.1007/BF00331449. [DOI] [PubMed] [Google Scholar]
  22. Subramanian A. R. Copies of proteins L7 and L12 and heterogeneity of the large subunit of Escherichia coli ribosome. J Mol Biol. 1975 Jun 15;95(1):1–8. doi: 10.1016/0022-2836(75)90330-7. [DOI] [PubMed] [Google Scholar]
  23. Watson R. J., Parker J., Fiil N. P., Flaks J. G., Friesen J. D. New chromosomal location for structural genes of ribosomal proteins. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2765–2769. doi: 10.1073/pnas.72.7.2765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. van Ooyen A. J., Gruber M., Jorgensen P. The mechanism of action of ppGpp on rRNA synthesis in vitro. Cell. 1976 May;8(1):123–128. doi: 10.1016/0092-8674(76)90193-8. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES