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. 1973 Jan;70(1):270–274. doi: 10.1073/pnas.70.1.270

Development of Acetylcholine Receptor Clusters on Cultured Muscle Cells

A J Sytkowski 1, Z Vogel 1, M W Nirenberg 1
PMCID: PMC433229  PMID: 4509661

Abstract

Clusters of acetylcholine receptors were formed in the absence of neurons during the development of cultured chick-embryo skeletal muscle cells. The average concentration of receptors in clusters was estimated to be 9000 per μm2. At other regions of the cell, receptor concentration was 900 per μm2. The possibility that receptor clusters may participate in synapse formation is suggested.

Keywords: α-bungarotoxin, autoradiography, synapses

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Selected References

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  1. AXELSSON J., THESLEFF S. A study of supersensitivity in denervated mammalian skeletal muscle. J Physiol. 1959 Jun 23;147(1):178–193. doi: 10.1113/jphysiol.1959.sp006233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnard E. A., Wieckowski J., Chiu T. H. Cholinergic receptor molecules and cholinesterase molecules at mouse skeletal muscle junctions. Nature. 1971 Nov 26;234(5326):207–209. doi: 10.1038/234207a0. [DOI] [PubMed] [Google Scholar]
  3. Berg D. K., Kelly R. B., Sargent P. B., Williamson P., Hall Z. W. Binding of -bungarotoxin to acetylcholine receptors in mammalian muscle (snake venom-denervated muscle-neonatal muscle-rat diaphragm-SDS-polyacrylamide gel electrophoresis). Proc Natl Acad Sci U S A. 1972 Jan;69(1):147–151. doi: 10.1073/pnas.69.1.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bosmann H. B. Acetylcholine receptor. I. Identification and biochemical characteristics of a cholinergic receptor of guinea pig cerebral cortex. J Biol Chem. 1972 Jan 10;247(1):130–145. [PubMed] [Google Scholar]
  5. Bourgeois J. -P., Ryter A., Menez A., Fromageot P., Boquet P., Changeux J. -P. Localization of the cholinergic receptor protein in Electrophorus electroplax by high resolution autoradiography. FEBS Lett. 1972 Sep 1;25(1):127–133. doi: 10.1016/0014-5793(72)80469-1. [DOI] [PubMed] [Google Scholar]
  6. Changeux J. P., Kasai M., Lee C. Y. Use of a snake venom toxin to characterize the cholinergic receptor protein. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1241–1247. doi: 10.1073/pnas.67.3.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DIAMOND J., MILEDI R. A study of foetal and new-born rat muscle fibres. J Physiol. 1962 Aug;162:393–408. doi: 10.1113/jphysiol.1962.sp006941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fambrough D. M., Hartzell H. C. Acetylcholine receptors: number and distribution at neuromuscular junctions in rat diaphragm. Science. 1972 Apr 14;176(4031):189–191. doi: 10.1126/science.176.4031.189. [DOI] [PubMed] [Google Scholar]
  10. Fambrough D., Rash J. E. Development of acetylcholine sensitivity during myogenesis. Dev Biol. 1971 Sep;26(1):55–68. doi: 10.1016/0012-1606(71)90107-2. [DOI] [PubMed] [Google Scholar]
  11. Fulpius B., Cha S., Klett R., Reich E. Properties of the nicotinic acetylcholine receptor macromolecule of Electrophorus electricus. FEBS Lett. 1972 Aug 15;24(3):323–326. doi: 10.1016/0014-5793(72)80382-x. [DOI] [PubMed] [Google Scholar]
  12. Harris A. J., Dennis M. J. Acetylcholine sensitivity and distribution on mouse neuroblastoma cells. Science. 1970 Feb 27;167(3922):1253–1255. doi: 10.1126/science.167.3922.1253. [DOI] [PubMed] [Google Scholar]
  13. KATZ B., MILEDI R. THE DEVELOPMENT OF ACETYLCHOLINE SENSITIVITY IN NERVE-FREE SEGMENTS OF SKELETAL MUSCLE. J Physiol. 1964 Mar;170:389–396. doi: 10.1113/jphysiol.1964.sp007339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Konigsberg I. R. Diffusion-mediated control of myoblast fusion. Dev Biol. 1971 Sep;26(1):133–152. doi: 10.1016/0012-1606(71)90113-8. [DOI] [PubMed] [Google Scholar]
  15. Kuffler S. W., Dennis M. J., Harris A. J. The development of chemosensitivity in extrasynaptic areas of the neuronal surface after denervation of parasympathetic ganglion cells in the heart of the frog. Proc R Soc Lond B Biol Sci. 1971 Apr 27;177(1049):555–563. doi: 10.1098/rspb.1971.0047. [DOI] [PubMed] [Google Scholar]
  16. Lee C. Y., Tseng L. F., Chiu T. H. Influence of denervation on localization of neurotoxins from clapid venoms in rat diaphragm. Nature. 1967 Sep 9;215(5106):1177–1178. doi: 10.1038/2151177a0. [DOI] [PubMed] [Google Scholar]
  17. MILEDI R. FORMATION OF EXTRA NERVE-MUSCLE JUNCTIONS IN INNERVATED MUSCLE. Nature. 1963 Sep 21;199:1191–1192. doi: 10.1038/1991191a0. [DOI] [PubMed] [Google Scholar]
  18. MILEDI R. The acetylcholine sensitivity of frog muscle fibres after complete or partial devervation. J Physiol. 1960 Apr;151:1–23. [PMC free article] [PubMed] [Google Scholar]
  19. Meunier J. C., Olsen R., Menez A., Morgat J. L., Fromageot P., Ronseray A. M., Boquet P., Changeux J. P. Quelques propriétés physique de la protéine réceptrice de l'acétylcholine étudiées à l'aide d'une neurotoxine radioactive. C R Acad Sci Hebd Seances Acad Sci D. 1971 Aug 2;273(5):595–598. [PubMed] [Google Scholar]
  20. Miledi R., Molinoff P., Potter L. T. Isolation of the cholinergic receptor protein of Torpedo electric tissue. Nature. 1971 Feb 19;229(5286):554–557. doi: 10.1038/229554a0. [DOI] [PubMed] [Google Scholar]
  21. Miledi R., Potter L. T. Acetylcholine receptors in muscle fibres. Nature. 1971 Oct 29;233(5322):599–603. doi: 10.1038/233599a0. [DOI] [PubMed] [Google Scholar]
  22. Patrick J., Heinemann S. F., Lindstrom J., Schubert D., Steinbach J. H. Appearance of acetylcholine receptors during differentiation of a myogenic cell line. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2762–2766. doi: 10.1073/pnas.69.10.2762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Raftery M. A., Schmidt J., Clark D. G. Specificity of -bungarotoxin binding to Torpedo californica electroplax. Arch Biochem Biophys. 1972 Oct;152(2):882–886. doi: 10.1016/0003-9861(72)90285-8. [DOI] [PubMed] [Google Scholar]
  24. Vogel Z., Sytkowski A. J., Nirenberg M. W. Acetylcholine receptors of muscle grown in vitro. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3180–3184. doi: 10.1073/pnas.69.11.3180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vogt M., Dulbecco R. VIRUS-CELL INTERACTION WITH A TUMOR-PRODUCING VIRUS. Proc Natl Acad Sci U S A. 1960 Mar;46(3):365–370. doi: 10.1073/pnas.46.3.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. White N. K., Hauschka S. D. Muscle development in vitro. A new conditioned medium effect on colony differentiation. Exp Cell Res. 1971 Aug;67(2):479–482. doi: 10.1016/0014-4827(71)90437-x. [DOI] [PubMed] [Google Scholar]

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